突触融合蛋白8在脑胶质瘤组织中的表达及临床意义

Expression of syntaxin 8 in glioma tissue and its clinical significance

目的:观察脑胶质瘤组织中突触融合蛋白8(syntaxin 8,STX8)的表达情况,并探讨其与病理分级的关系。方法:收集北京市仁和医院2013年5月至2015年12月进行手术治疗的脑胶质瘤患者57例,将术后脑胶质瘤组织(57例)作为观察组,其中Ⅰ+Ⅱ级12例(低级别)、Ⅲ+Ⅳ级45例(高级别),另取正常脑组织(15例)作为对照组。采用Real-time PCR检测STX8 m RNA的表达,免疫组织化学染色、Western blot方法检测STX8的蛋白表达,分析STX8的表达与脑胶质瘤临床病理的关系。结果:与正常脑组织相比,脑胶质瘤组织中STX8的m RNA表达明显升高,其中在低级别、高级别脑胶质瘤组织中相对表达量分别为:1.685 5±0.071 24、2.820 7±0.069 2,两组相比,且分别与对照组(1±0.063 6)相比,差异均有统计学意义(P<0.05)。免疫组织化学结果显示,脑胶质瘤组织中STX8高表达,在正常脑组织中表达量较低。Western Blot结果显示STX8蛋白表达水平明显高于正常脑组织(P<0.05);其中在低级别、高级别脑胶质瘤组织中STX8蛋白的相对表达量分别为2.271±0.1621、4.937±0.1851,两组相比,且分别与对照组相比,差异均有统计学意义(P<0.05)。统计学分析发现STX8在脑胶质瘤组织中的高表达与患者的性别、年龄及病理分型无关,但在不同的病理分期之间差异极显著(P<0.01)。结论:STX8在脑胶质瘤组织中异常高表达,可能与脑胶质瘤的发生发展密切相关。

Objective To investigate the expression of syntaxin 8 （STX8） in glioma and its clinical signif- icance. Methods Specimens of glioma were collected from 57 patients at Beijing Renhe Hospital from May 2013 to December 2015. 57 pieces of glioma tissue were used as a study group, 12 of which were Ⅰ ＋ Ⅱ （low grade） and the rest 45 were Ⅲ ＋Ⅳ ; normal brain tissues from 15 individuals were used as a control group. Real-time PCR, immunohistochemistry, and Western blot were used to detect expression of STX8. Results As compared with the normal brain tissue, the mRNA expression of STX8 was significantly increased in glioma tissue, with a relative expression volume of 1.685 5± 0.0712 4 in low grade and 2.820 7 ± 0.069 2 in high grade tissues, there was significant differences between the two groups; and the difference was also significant as compared with the control group （P 〈 0.05 ）. The results of immunohistoehemistry showed that the expression of STX8 was higher in glioma tissue than in normal tissue. Western Blot showed that the expression of STX8 protein was significantly higher in glioma than in normal tissue （P 〈 0.05） ; the relative expression volume of STX8 was 2.271 ± 0.162 1 in low grade tissue and 4.937 ± 0.185 1 in high grade tissue, with a significant difference between the two groups; the difference was also significant as compared with the control group （P 〈 0.05）. The correlation analysis showed that higher STX8 expression in glioma was not significantly related to gender, age and pathological types, but there was a significant difference between pathological stages. Conclusion STX8 has abnormal high expression in glioma, which may be closely related with the occurrence and development of glioma.