乙醇及洗必泰乙醇溶液破坏乙型肝炎表面抗原作用的观察

OBSERVATION ON DESTRUCTIVE EFFECT OF ETHANOL AND ETHANOL-CHLORHEXIDINE SOLUTION ON HBsAg

实验室试验表明,乙醇对患者血清中HBsAg具有一定破坏作用,当加入洗必泰时,破坏作用有所加强。乙醇溶液在有盖玻璃瓶中保存45天,浓度变化不大,但存放时间愈长,容器敞口愈大,浓度下降愈多。

The destructive effect of ethanol on HBsAg in patient's serum was examined in laboratory by means of SPRIA . In 1: 10 diluted serum, 75% and 80% ethanol with a 30 min contact time could not destroy completely the HBsAg in samples of 5 experiments , which became negative in all of the 5 experiments only when the serum of patient was diluted to 1:500.70% ethanol was less effective than 75% or 80% ethanol in destroying HBsAg. The destructive effect on HBsAg in serum was potentiated when chlorhexidine was added to the ethanol. For example, when diluted (1:10) patient's serum was exposed to the combination of 75% ethanol and 0.5% chlorhexidine for 15 min, no HBsAg could be detected by SPRIA and immunological electron microscopic examination was also negative.