摘要
采用硅胶柱层析、ODS反相硅胶柱层析、凝胶柱层析和高效液相HPLC等色谱技术,对极地真菌Geomyces sp.3-1的发酵液提取物进行分离纯化,共得到8个化合物,通过波谱解析结合理化性质并比较相关文献,确定化合物为:paulownin(1)、demethylincisterol A3(2)、ergosta-7,22-dienen-3,6-dione(3)、citreoanthrasteroid B(4)、19-norergosta-5,7,9,22-tetraene-3β-ol(5)、(22E)-5α,8α-epidioxyergosta-6,22-dien-3β-ol(6)、(3β,5α,6β,22E)-6-methoxyergosta-7,22-diene-3,5-diol(7)和ergosta-7,22-dien-3β-ol(8)。其中,化合物2~7均是首次从Geomyces属真菌中获得,化合物2有较好的抗菌和细胞毒活性。
Eight compounds were obtained from the fermentation of the polar-derived fungus Geomyces sp. 3-1. The ex- tract was isolated and purified by column chromatography on silica-gel, reversed phase chromatography, Sephadex LH-20 and HPLC. The structures of compounds were elucidated by physical and chemical properties and spectral analysis, and comparison with the data of literatures. The eight compounds were identified as panlownin (1), demethylincisterol A3 ( 2), ergosta-7,22-dienen-3,6-dione ( 3 ), citreoanthrasteroid B (4), 19-norergosta-5,7,9,22-tetraene-3β-ol ( 5 ), (22E) - 5α,8α-epidioxyergosta-6,22-dien-3β-ol(6) ,(3β,5α,6β,22E)-6-methoxyergosta-7,22-diene-3,5-diol(7) and ergosta- 7,22-dien-3β-ol(8). Compounds 2-7 were firstly isolated from Geomycesspecies,and compound 2 was shown to have an- tibacterial and eytotoxic activities.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第4期590-594,共5页
Natural Product Research and Development
基金
广东省海洋局公益项目(GD2012-D01-001)
国家自然科学基金(41306197
41606173)
关键词
极地真菌
地丝霉属
次级代谢产物
生物学活性
polar-derived fungus
Geomyces sp.
secondary metabolites
biological activity