极地真菌Geomyces sp.3-1次级代谢产物的研究

Secondary Metabolites of Polar-Derived Fungus Geomyces sp. 3-1

采用硅胶柱层析、ODS反相硅胶柱层析、凝胶柱层析和高效液相HPLC等色谱技术,对极地真菌Geomyces sp.3-1的发酵液提取物进行分离纯化,共得到8个化合物,通过波谱解析结合理化性质并比较相关文献,确定化合物为:paulownin(1)、demethylincisterol A3(2)、ergosta-7,22-dienen-3,6-dione(3)、citreoanthrasteroid B(4)、19-norergosta-5,7,9,22-tetraene-3β-ol(5)、(22E)-5α,8α-epidioxyergosta-6,22-dien-3β-ol(6)、(3β,5α,6β,22E)-6-methoxyergosta-7,22-diene-3,5-diol(7)和ergosta-7,22-dien-3β-ol(8)。其中,化合物2~7均是首次从Geomyces属真菌中获得,化合物2有较好的抗菌和细胞毒活性。

Eight compounds were obtained from the fermentation of the polar-derived fungus Geomyces sp. 3-1. The ex- tract was isolated and purified by column chromatography on silica-gel, reversed phase chromatography, Sephadex LH-20 and HPLC. The structures of compounds were elucidated by physical and chemical properties and spectral analysis, and comparison with the data of literatures. The eight compounds were identified as panlownin （1）, demethylincisterol A3 （ 2）, ergosta-7,22-dienen-3,6-dione （ 3 ）, citreoanthrasteroid B （4）, 19-norergosta-5,7,9,22-tetraene-3β-ol （ 5 ）, （22E） - 5α,8α-epidioxyergosta-6,22-dien-3β-ol（6） ,（3β,5α,6β,22E）-6-methoxyergosta-7,22-diene-3,5-diol（7） and ergosta- 7,22-dien-3β-ol（8）. Compounds 2-7 were firstly isolated from Geomycesspecies,and compound 2 was shown to have an- tibacterial and eytotoxic activities.