NIRS快速测定河南产不同加工方法野菊花中木犀草素的含量

Quantitative Analysis of Luteolin in Chrysanthemum indicum Originating from Henan by Near-infrared Spectroscopy

本文利用近红外光谱技术(NIRS)快速测定河南产不同加工方法野菊花药材中木犀草素的含量。采用3种加工方法(直接晒干、蒸后晒干和炒后晒干)对河南新郑、三门峡和信阳三个不同产地的野菊花药材进行炮制加工,采用高效液相色谱法(HPLC)测定野菊花药材中木犀草素的含量,采集野菊花药材的NIRS图谱,运用偏最小二乘法(PLS)建立河南不同产区不同加工方法野菊花药材中木犀草素的定量分析模型。结果表明所建立的木犀草素定量分析模型的内部交叉验证相关系数(R2)、校正均方差(RMSEC)、预测均方差(RMSEP)分别为0.94525、0.0142、0.0428;验证集样品的木犀草素含量近红外预测值的平均相对偏差为2.95%,预测性能良好。该方法测定高效、快捷、准确度高,可用于河南产不同加工方法的野菊花药材中木犀草素含量的快速测定。

Near-infrared spectroscopy method was established for the rapid determination of luteolin in Chrysanthemum indicum made by different processing methods and from different producing areas of Henan. Three manufacturing meth- ods （drying directly, drying after steaming, drying after firing） were adopted to process wild chrysanthemum flower from Xinzheng, Sanmenxia and Luoyang, respectively. The content of luteolin in the C. indicum was measured by HPLC. The NIRS spectra of wild Chrysanthemum were collected. Then, the near-infrared spectroscopy was combined with PLS analy- sis to establish the quantitative model of luteolin in C. indicum. The correlation coefficients（R2 ）, root mean square error of calibration（RMSEC） and the root mean square error of predication（RMSEP） of the established quantitative analysis model of luteolin were 0. 94525,0. 0142 and 0. 0428, respectively. The near infrared prediction correlation coefficient of luteolin content in validation sets samples was 2.95% ,which suggested good quality of prediction. The method was effi- cient,rapid and accurate, and can be used for the rapid determination of the content of luteolin in C. indicum from nenan.