大孔吸附树脂法去除山银花总皂苷的工艺研究

Removal Technology of Total Saponins from Lonicerae Flos by Macroporous Resin

本文采用大孔吸附树脂法对山银花总皂苷的去除工艺进行了研究。以山银花总皂苷含量为指标,比较了4种大孔树脂对山银花总皂苷的吸附和解吸附性能,并对优选出的树脂进行吸附工艺参数的优化。结果表明,HPD-100型大孔树脂具有较好的吸附性能,其最佳吸附工艺条件为:药液上样浓度0.65 g/m L,流速2 m L/min,药液p H值为4,吸附12 h。经蒸馏水和30%乙醇洗脱各5 BV后,收集两者的洗脱液,总皂苷去除率和总咖啡酰奎宁酸富集率均接近90%,确定了本实验所建立的方法能有效去除山银花总皂苷,富集总咖啡酰奎宁酸。

This study focused on the removal of total saponins and the enrichment of total caffeoylquinic acids from Lonicerae Flos using macroporous adsorption resin. The adsorption and desorption capacities for total saponins of 4 resins were compared. The adsorption characters, the elution parameters and the influencing factors of the selected macroporous resin were optimized. The results demonstrated that HPD-100 macroporous resin possessed superior adsorptive and des- orptive properties on total saponins than the other 3 resins. The optimized conditions were as follow~;the ~ample concen- tration was 0. 65 g/mL,the flow rate was 2 mL/min,the pH value was 4,the adsorption time was 12 h,and the elution reagents were 5 BV of water and 5 BV of 30% ethanol. Under the optimized conditions, both the removal ratio of total saponins and enrichment ratio of total caffeoylquinic acids were closed to 90% , indicating that the removal technology of this study can significantly decrease the content of total saponins and enrich the caffeoylquinic acids in Lonicerae Flos.