消渴平合剂对链脲佐菌素诱导的糖尿病肾病大鼠肾功能及miR-192的影响研究

Research of Xiaokeping Mixture on Kideny Function and miR-192 of STZ-induced Diabetic Nephropathy Rats

目的:观察消渴平合剂对链脲佐菌素(STZ)诱导的糖尿病肾病(DN)大鼠肾功能及肾组织miR-192的影响,探讨其对DN的防治作用及机制。方法:采用高脂高糖饮食联合STZ腹腔注射构建DN大鼠模型,随机分为正常组,模型组、厄贝沙坦组和消渴平合剂组。16周时处死大鼠,检测各组大鼠糖化血红蛋白、24 h尿蛋白、血肌酐(Scr)、尿素氮(BUN)、肾重(KW)、相对肾重的变化,RT—PCR检测大鼠肾组织miR-192的变化。结果:消渴平合剂可以改善DN大鼠的一般状况,与模型组比较,消渴平合剂组糖化血红蛋白、24 h尿蛋白定量、Scr、BUN含量显著降低(P<0.05),肾重及相对肾重明显减降低(P<0.05)。与厄贝沙坦组相比,消渴平合剂组糖化血红蛋白、24 h尿蛋白显著降低(P<0.05)。消渴平合剂组及厄贝沙坦组大鼠肾组织miR-192含量均较模型组下调(P<0.05),消渴平合剂组与厄贝沙坦组比较无显著性差异(P>0.05)。结论:消渴平合剂可用于治疗糖尿病肾病,其机制可能与抑制DN大鼠肾组织miR-192的激活有关。

Objective ： To explore the mechanism of Xiaokeping Mixture on diabetic nephropathy rats （ DN rats） induced by streptozotocin（STZ） and the expression of miR - 192 of kidney tissues. Methods： The experimental rats were induced by high fat and high sugar diet in combination with small dose of STZ. The rats were randomly divided into four groups ： the normal control group, the model group, the Irbesartan group and the Xiaokeping Mixture group. Some biochem- ical indicators were observed. Rats were executed on the 16th week. The glycosylated hemoglobin, urine protein in 24 h, BUN,Scr,kidney weight and relative kidney weight were tested. The expressions of miR - 192 were detected by RT - PCR. Results ： The general condition of rats could be improved by Xiaokeping Mixture. Compared with the model group, the glycosylated hemoglobin, 24 h urinary protein and the contents of BUN, Scr, kidney weight, relative kidney weight in Xiaokeping Mixture group could be obviously reduced（P 〈 0. 05）. Compared with the Irbesartan group, the glycosylated hemoglobin and 24 h urinary protein were significantly reduced in the Xiaokeping Mixture group（ P 〈 0. 05 ）. The expressions of miR- 192 were significantly reduced in the Irbesartan group and Xiaokeping Mixture group（ P 〈 0. 05 ）, while there were no significant differences between Xiaokeping Mixture high dose group and Irbesartan group（ P 〉 0. 05 ）. Conclusion： It was found that Xiaokeping Mixture could be used to treat DN. The mechanism may be related to restraining the activation of miR - 192.