卵巢浆液性癌组织中microRNA表达谱分析

Expression Profile of MicroRNA in Ovarian Serous Carcinoma

目的寻找与卵巢浆液性癌相关的microRNA,积累以国内患者为数据来源的microRNA表达谱资料,并初步探讨其临床意义。方法利用microRNA芯片,筛查卵巢浆液性癌组织及正常卵巢组织中差异表达的microRNA,用实时荧光定量PCR在58份卵巢组织标本中进行验证,并分析microRNA表达水平与卵巢浆液性癌临床病理特征之间的关系。结果与正常卵巢组织比较,芯片结果显示卵巢浆液性癌有9个microRNA明显上调,分别是hsa-mi R-141-3p、hsa-mi R-200a-3p、hsa-mi R-200b-3p、hsa-mi R-200c-3p、hsa-mi R-224-5p、hsa-mi R-7-5p、hsa-mi R-142-3p、hsa-mi R-21-5p、hsa-mi R-142-5p,6个microRNA明显下调,分别是hsa-mi R-424-5p、hsa-mi R-214-3p、hsa-mi R-125b-5p、hsa-let-7b-5p、hsa-mi R-199a-5p、hsa-mi R-100-5p。实时荧光定量PCR验证结果与芯片结果一致。对差异microRNA进行分层分析发现,与卵巢浆液性癌Ⅰ-Ⅱ期、淋巴结未转移患者比较,Ⅲ-Ⅳ期、淋巴结有转移患者的hsa-mi R-21-5p表达均增高,hsa-mi R-125b-5p表达均降低(P<0.05);而在高级别与低级别卵巢癌患者之间microRNA的表达水平差异均无统计学意义(P均>0.05)。结论卵巢浆液性癌中异常表达的microRNA可能与肿瘤的发生发展相关。

Objective To search for microRNA associated with ovarian serous carcinoma, and to accumulate the microRNA expression profile data from domestic patients, and to make a preliminary study of its clinical significance. Methods Microarray was used to screen the differentially expressed microRNA in ovarian serous carcinoma and normal ovarian tissues, and the real-time fluorescence quantitative PCR was used to verify the expression in 58 samples of ovarian tissues. The relationship between microRNA expression and clinicopathological features of ovarian serous carcinoma was analyzed. Results Compared with the normal ovarian tissue, nine microRNA were up-regulated in ovarian serous carcinoma by microarray and they were hsa-miR-141-3p, hsa-miR-200a-3p,hsa-miR-200b-3p,hsa-miR-200c-3p,hsa-miR-224-Sp, hsa-miR-7-Sp, hsa-miR- 142- 3p, hsa-miR-21-Sp,hsa-miR-142-Sp, respectively. Six microRNA was significantly down-regnlated and they werehsa-miR-424-Sp, hsa-miR-214-3p, hsa-miR- 125b-Sp, let-7b-Sp, hsa-miR- 199a-Sp, hsa-miR- 100- 5p, respectively. The results of real-time fluorescence quantitative PCR were consistent with the chips. And in the stratified analysis of different microRNA,compared with no lymph node metastasis and I-II stage, hsa-miR-21-5p expression increased in III-IV stage lymph node metastasis patients, and hsa-miR-125b-Sp expression decreased （P〈0.05）. Conclusion The abnormal expression of microRNA in ovarian serous carcinoma is related to the occurrence and development of tumor.