陕西佛坪“刷把菌”子实体多糖提取工艺及抗菌活性

Extraction and antimicrobial activity of “Shuaba mushroom” polysaccharide from Foping,Shaanxi

【目的】优化陕西佛坪"刷把菌"子实体多糖提取工艺并研究其多糖抗菌活性,为其多糖提取的中试生产和综合利用提供理论依据。【方法】以陕西佛坪"刷把菌"为材料,采用水浸提法和苯酚-硫酸法提取粗多糖并测定其含量,以浸提时间、浸提温度、液料比和浸提次数为影响因素,以多糖提取率为考察指标,在单因素试验基础上,采用L9(34)正交试验对提取工艺条件进行优化,滤纸片法测定粗多糖的抗菌活性。【结果】陕西佛坪"刷把菌"子实体多糖提取最佳工艺是浸提时间3h,浸提温度90℃,液(mL)料(g)比15∶1,浸提次数3次,在此条件下,多糖提取率是(4.73±0.016)%。提取的多糖对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、铜绿杆菌、白色念珠菌和沙门氏菌均有抗菌活性。【结论】得到了陕西佛坪"刷把菌"子实体多糖的优化提取工艺,提取出的多糖具有抗菌活性。

[Objective] Extraction process of polysaccharide from ＂Shuaba mushroom＂ in Foping, Shaanxi was optimized and its antimicrobial activity was determined to provide theoretical basis for the in- dustrial production and comprehensive utilization of polysaccharide from Ramaria botrytoides. [Method] Based on single factor test, the influencing factors is extracting time, temperature, ratio of liquid to solid and times, the indexes is extraction rat,extraction conditions for polysaccharide from ＂Shuaba mushroom＂ from Foping,Shaanxi were optimized by L9 （3^4） orthogonal design. Its antimicrobial activity was also de- termined using filter paper method. [Result] The optimal conditions obtained by L9 （3^4） orthogonal design were： extraction time 3 h,extraction temperature 90 ℃ ,ratio of liquid to solid（mL/g） 15 ： 1, and extrac- tion by 3 times. Under these conditions,the extraction yield of polysaccharide was up to （4.73±0. 016）%. The polysaccharide from ＂Shuaba mushroom＂ had antimicrobial activities against Escherichia coli, P. aeruginosa , Salmonella, Bacillus subtilis , Staphylococcus aureus , and Monilia albican. [Conclusion] The study obtained the optimal process of ＂Shuaba mushroom＂ in Foping, Shaanxi and the extracted crude poly- saccharide had antibacterial activity.