载药荧光纳米粒子的制备及在乳腺癌MCF-7细胞系的应用及效果评价

Fabrication of Drug Loaded Fluorescent Nanoparticles and Its Biological Application in MCF-7 Breast Cancer Cell

利用两亲性聚乙二醇-聚乳酸共聚物(PEG-PDLLA)包覆荧光染料(DPBA)和紫杉醇(PTX),通过自组装方法制得载药荧光纳米粒子DPBA/PTX@PEG-PDLLA.纳米粒子尺寸均一,具有良好的生物相容性.对纳米粒子的发光性质、载药量和体外药物释放等进行了表征,并考察了纳米粒子对乳腺癌细胞MCF-7的抑制效果,观察了MCF-7细胞对纳米粒子的摄取情况.结果表明,DPBA/PTX@PEG-PDLLA纳米粒子具有较强的红光发射,不仅可以用于MCF-7肿瘤细胞质荧光成像,而且对肿瘤细胞的增殖具有一定的抑制能力.

Drug-loaded fluorescent nanoparticles（ DPBA/PTX@ PEG-PDLLA） were prepared by amphiphilic polymers（PEG-PDLLA） coated with AIE dyes （DPBA） and paclitaxel （PTX）. The effects of capacity of DPBA and PTX on the photophysical properties of nanoparticles were investigated. In addition, the drug release in vitro of nanoparticles as well as the inhibitory effect of nanoparticles on breast cancer MCF-7 cells were investigated. Moreover, the nanoparticle uptake of MCF-7 cells was observed. As conclusion, the DPBA/ PTX@ PEG-PDLLA nanoparticles exhibit strong red emission（654 nm） with high fluorescence quantum yield up to 25%. The nanoparticles have uniform size and good biocompatibility. The freshly prepared nanoparticles have pretty drug release ability, and the cumulative release rate of 48 h can reach 25.1%. The results of in vitro MTT and CLSM experiments show that DPBA/PTX@ PEG-PDLLA nanoparticles not only have good inhibiting ability with the cell proliferation of MCF-7 tumor cells, but also can be uptake by tumor cell and then absorbed by their cytoplasmic fluorescence imaging.