高效抗菌Cu^(2+)-壳聚糖季铵盐/丙烯酸复合物的制备

Preparation of Cu^(2+)-Chitosan Quaternary Ammonium Salt-g-Poly(acrylicacid) with Efficient Antibacterial Activity

以过硫酸铵为引发剂,在壳聚糖季铵盐(HACC)上接枝丙烯酸(AAc)并络合Cu^(2+),制备了具有高效抗菌性能的HACC-g-PAAc-Cu^(2+)复合物.采用红外光谱(FTIR)和核磁共振波谱(~1H NMR)表征了壳聚糖季铵盐接枝改性前后的化学结构变化;利用紫外-可见吸收光谱(UV-Vis)、Cu^(2+)选择电极和热失重分析(TGA)表征了壳聚糖季铵盐接枝前后负载Cu^(2+)的能力;测定了HACC-Cu^(2+)和HACC-g-PAAc-Cu^(2+)对金黄色葡萄球菌及大肠杆菌的最小抑菌浓度,并对小鼠经口摄入毒性和兔皮肤敷涂刺激性进行了考察.研究结果表明,在壳聚糖季铵盐上负载Cu^(2+)能够有效提高其抗菌性;接枝丙烯酸能提高HACC负载Cu^(2+)的能力和抗菌性,Cu/HACC结构单元的摩尔比由接枝前的3∶7提高到接枝后的1∶1;对金黄色葡萄球菌的最小抑菌浓度由60 mg/L下降到9.2 mg/L,对大肠杆菌的最小抑菌浓度由37 mg/L下降到6.3 mg/L,无摄入毒性和皮肤刺激性.

Acrylic acid （AAc） was grafted to chitosan quaternary ammonium salt （HACC） by ammonium persulfate as an initiator. HACC-g-PAAc-Cu2＋ with efficient antibacterial activity was further prepared through complexation of Cu2＋ with HACC-g-PAAc. The chemical structure of the grafted HACC was characterized by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance （1H NMR）. The capacity of grafted HACC loading Cu2＋ was determined by ultraviolet-visible spectroscopy（UV-Vis） and thermagravimetric analysis（TGA）. Antimicrobial property of HACC-Cu2＋ and HACC-g-PAAc-Cu2＋ against staphylococcus aurous （S. Aurous） and E. coli bacillus were tested. Toxicity for oral intake of mice and coating irritation on rabbit skin of HACC-Cu2＋ and HACC-g-PAAc-Cu2＋ were also evaluated. The property of antibacterial and capacity of complexing Cu2＋ can be improved by grafting of AAc. The molar ratio of Cu2＋/HACC increased from 3 ： 7 before grafting to 1 ： 1 after grafting. The minimum inhibitory concentration（MIC） of S. Aurous and E. coli bacil- lus decreased from 60 mg/L to 9. 2 mg/L and 37 mg/L to 6. 3 mg/L. Moreover, HACC-Cu2＋ and HACC-g- PAAc-Cu2＋ have no toxic ingestion and skin irritation.