猪血抗凝剂的筛选与抗凝条件优化研究

Study on the Screening of Pig Blood Anticoagulant and Optimization of Anticoagulant Condition

[目的]筛选抗凝剂配方并优化抗凝条件。[方法]以新鲜猪血液为试材,以血液黏度、血红蛋白OD_(414nm)值、血红细胞数量为指标,研究添加不同抗凝剂后的猪血理化与生化特性。[结果]抗凝效果最佳的抗凝剂组合与条件为柠檬酸钠100%,pH 9.19,添加量6 g/L,4℃冷藏保存;或六偏磷酸钠100%,pH 1.76,添加量4 g/L,常温或4℃保存。[结论]对比该试验与他人研究结果,选用单一或复合型抗凝剂有待进一步探讨。

[ Objective ] To screen and optimize the anticoagulant formula and condition. [ Method ] Taking fresh pig blood as test material and blood viscosity, OD414nm value as well as blood cell count as main indexes, physicochemical and biochemical characteristics of pig blood with addition of different anticoagulants were measured. [ Result] Conditions and formula with best anticoagulant effect was 100% of sodium citrate, pH 9.19, adding amount 6 g/L and stored in 4 ℃, or sodium hexametaphosphate 100%, pH 1.76, adding amount 4 g/L and stored at room temperature or in 4℃. [ Conclusion] Comparing experiments results got in this paper with others, using single or compound anticoagulant needs further study.