黔产野生水芹水溶性总黄酮纯化工艺

Purification Process of Water-Soluble Total Flavonoids from Wild Oenanthe javanica in Guizhou Province

[目的]考察大孔树脂对黔产野生水芹(简称水芹)水溶性总黄酮的纯化条件。[方法]测定AB-8、DM-130和HP-20 3种大孔树脂对水芹总黄酮的吸附量及解吸率,筛选树脂型号;以总黄酮含量为指标,采用单因素试验优选HP-20树脂纯化工艺。[结果]HP-20型大孔树脂分离纯化水芹总黄酮最佳的工艺条件为:料液比为1∶30(g∶mL),静置吸附时间为6 h,解吸液乙醇溶液的体积分数为70%,pH为3,解吸温度为40℃,经HP-20处理后的水芹总黄酮纯度较高(提高了9.4倍)。[结论]HP-20树脂较适于分离纯化黔产野生水芹水溶性总黄酮,为水芹的开发利用提供科学依据。

[ Objective] The research aimed to study the purification conditions of water-soluble total flavonoids from wild Oenanthe javanica in Guizhou Province. [ Method ] The adsorption quantity and desorption rate of three kinds of macroporous resins （AB -8, DM - 130 and HP -20） were measured for total flavonoids of Oenanthe javanica, resin type was selected. Using the content of total flavonoids as index, the single factor experiment was used to optimize the purification process of HP- 20 resin. [ Result] The optimum process conditions of separation and purification of total flavonoids from Oenanthe javanica for HP- 20 macroporous resin：solid-liquid ratio was 1：30 （g： mL）, standing time was 6 h, the volume fraction of the desorption ethanol solution was 70% ,pH value of sample was 3 ,the desorption temperature was 40 ~C o The total flavonoids had high purity after HP -20 maeroporous resin （increased 9.4 times）. [ Conclusion] HP -20 resin is suitable for separation and purification of wa- ter-soluble total flavonoids,to provide a scientific basis for the development and utilization of Oenanthe javanica.