神经病理性疼痛大鼠模型接受免疫球蛋白干预后疼痛程度及神经炎症反应程度的分析

Analysis of the pain and neuroinflammation after rats with neuropathic pain receive immunoglobulin intervention

目的:研究免疫球蛋白干预对神经病理性疼痛大鼠疼痛程度及神经炎症反应程度的影响。方法:选择Wistar大鼠作为实验动物并随机分为假手术组(Sham组)、神经病理性疼痛模型组(NP组)、免疫球蛋白干预组(IG组),NP组和IG组建立神经病理性疼痛大鼠模型,IG组给予0.4g/kg免疫球蛋白干预。干预后21d时,测定血清及脊髓中疼痛介质、促炎因子、抗炎因子的含量。结果:NP组大鼠血清和脊髓中SP、5-HT、GABA、IFN-γ、TNF-α、IL-1β的含量显著高于Sham组,血清和和脊髓中IL-4、IL-10的含量显著低于Sham组;IG大鼠血清和脊髓中SP、5-HT、GABA、IFN-γ、TNF-α、IL-1β的含量显著低于NP组,血清和和脊髓中IL-4、IL-10的含量显著高于NP组。结论:免疫球蛋白能够减轻神经病理性疼痛大鼠的疼痛程度并抑制神经炎症反应程度。

Objective：To study the effect of immunoglobulin intervention on the pain and neuroinflammation in rats with neuropathic pain.Methods：Wistar rats were selected as experimental animals and randomly divided into control group（Sham group）,neuropathic pain model group（NP）and immunoglobulin intervention group（IG）,NP group and IG group were made into neuropathic pain rat models,and the IG group received 0.4g/kg immunoglobulin intervention.Twenty-one days after intervention,the levels of pain mediators,pro-inflammatory factors and anti-inflammatory factors in serum and spinal cord were detected.Results：Serum and spinal cord SP,5-HT,GABA,IFN-γ,TNF-αand IL-1βlevels of NP group were significantly higher than those of Sham group while serum and spinal cord IL-4and IL-10 levels were significantly lower than those of Sham group;serum and spinal cord SP,5-HT,GABA,IFN-γ,TNF-αand IL-1βlevels of IG group were significantly lower than those of NP group while serum and spinal cord IL-4and IL-10 levels were significantly higher than those of NP group.Conclusions：Immunoglobulin can relieve the pain and suppress the neuroinflammation in rats with neuropathic pain.