期刊文献+

SBR反应器厌氧氨氧化系统启动过程中细菌群落结构的变化 被引量:4

The shift of bacterial community structure during start-up of anaerobic ammonia oxidation(ANAMMOX) in a sequencing batch reactor
原文传递
导出
摘要 以白洋淀岸边带沉积物为接种污泥,启动了SBR厌氧氨氧化反应器.对反应器启动过程中的进出水水质进行了连续监测,并采用PCRDGGE、定量PCR和基因测序等分子生物学技术研究了系统内总细菌和厌氧氨氧化(ANAMMOX)细菌群落结构随培养时间的变化规律.结果表明:在启动过程中,总微生物菌群动态变化水平为26.6%~50.5%;微生物多样性先变小后增大,优势菌种得到重新分布;ANAMMOX细菌的群落结构变得单一化,最后系统的优势ANAMMOX细菌是Brocadia属.富集培养阶段SBR系统中ANAMMOX细菌的最大生物量达到了1.73×109copies·g^(-1)干污泥,而且总氮的去除率最高达到约82%. The start-up of anaerobic ammonia oxidation(ANAMMOX) system in a sequencing batch reactor(SBR),where Baiyangdian Lake shore sediments were inoculated.The microbial community structure of total bacteria and ANAMMOX bacteria in the system along with the enrichment time was investigated by using molecular biology methods(e.g.PCR-DGGE,q PCR,and DNA sequencing).Meanwhile,the water quality of the influent and effluent was continuously monitored during the whole start-up stage.The results showed that the dynamic change of total bacterial community along with the enrichment time increased from 26.6% to 50.5%,and their diversity decreased first and then increased afterwards.The ANAMMOX bacterial community diversity in the SBR became single at the end of enrichment,and was predominantly Candidutus Brocadia.The maximum biomass of ANAMMOX bacteria achieved 1.73 × 10^9 copies·g^-1 dry sludge during the enrichment,with the highest removal rate of TN around 82%.
出处 《环境科学学报》 CAS CSCD 北大核心 2017年第5期1695-1703,共9页 Acta Scientiae Circumstantiae
基金 中国人民大学科学研究基金(中央高校基本科研业务费专项资金资助)项目成果(No.15XNLD04)~~
关键词 白洋淀沉积物 厌氧氨氧化 SBR反应器 细菌多样性 群落结构 Baiyangdian sediments ANAMMOX SBR reactor bacterial diversity community structure
  • 引文网络
  • 相关文献

参考文献8

二级参考文献113

  • 1祖波,张代钧,卢培利,张萍,李小鹏.普通活性污泥富集好氧氨氧化菌试验[J].重庆大学学报(自然科学版),2005,28(2):100-103. 被引量:7
  • 2尹澄清,兰智文,晏维金.白洋淀水陆交错带对陆源营养物质的截留作用初步研究[J].应用生态学报,1995,6(1):76-80. 被引量:93
  • 3祖波,张代钧,白玉华,张萍.EGSB反应器中耦合厌氧氨氧化与甲烷化反硝化的研究[J].环境科学研究,2007,20(2):51-57. 被引量:30
  • 4国家环保局《水和废水监测分析方法》编委会.水和废水监测分析方法(第三版)[M].北京:中国环境科学出版社,1997.252-286.
  • 5汤洪霄.用水废水化学基础[M].北京:中国建筑工业出版社,1979.97-111.
  • 6万国江 章申 等.白洋淀的水质化学特征.白洋淀区域水污染控制研究(第一集)[M].北京:科学出版社,1995.32-35.
  • 7Hellinga C,Schellen A A J C,Mulder J W,et al. The SHARON process: an innovative method for nitrogen removal from ammonium-rich wastewater[J]. Wat Sci Tech, 1998,37(9) : 135-142.
  • 8Kuai L P,Verstraete W. Ammonium removal by the oxygen-limited autotrophic nitrificaiton-denitrification system [J].Appl Environ Microbiol, 1998,64( 11 ) :4500--4506.
  • 9Jetten M S M,Strous M,Katinka T,et al. The anaerobic oxidation of ammonium[J]. FEMS Microbiol Rev, 1999,22(6): 421-437.
  • 10Yang W, Vollertsen J, Hvitned-Jacobsen T. Nitrite accumulation in the treatment of wastewaters with high ammonia concentration[J].Wat Sci Tech, 2003,48(3 ) : 135-141.

共引文献129

同被引文献56

引证文献4

二级引证文献38

;
使用帮助 返回顶部