异丙酚对新生大鼠心肌细胞线粒体活性氧和细胞凋亡的影响

Effects of propofol on ROS and apoptosis in neonatal rat cardiomyocytes

目的:探讨异丙酚对线粒体介导的乳大鼠心肌细胞氧化应激通路的作用。方法:取新生大鼠乳鼠(3~4 d)心肌细胞作为研究对象,实验分为对照组(C组)、H_2O_2组(终浓度500μM,H组)、异丙酚组(50μM异丙酚,P组)、同时使用H_2O_2和异丙酚组(500μM H_2O_2+50μM异丙酚,PH组)。采用流式细胞分析仪测定异丙酚对大鼠乳鼠心肌细胞ROS水平的影响,使用ELISA法测定各组心肌细胞线粒体途径介导的凋亡因子Caspase 3和Caspase 9活性变化。结果 :异丙酚对H_2O_2介导的大鼠乳鼠心肌细胞ROS水平增加具有明显的抑制作用(P<0.05)。异丙酚可明显下调线粒体途径介导的凋亡因子Caspase 9和Caspase 3的活性(P<0.01)。结论:异丙酚通过抑制心肌细胞ROS水平以及下调线粒体介导的凋亡因子Caspase 9和Caspase 3的活性发挥对心肌细胞的保护作用。

Objective： This study aimed to investigate the effects of propofol on ROS and apoptosis of neonatal rat cardiomyocytes （NRCMs）. Methods： Cultured neonatal rat （3 ~ 4 days） cardiomyocytes were used in the study. The experiment included four groups： control group（Group C）, H2O2 group（final concentration of 500 μM, Group H）, propofol group（50 μM propofol, group P）, and H2O2 and propofol group（500 μM H2O2 ＋ 50 μM propofol, group PH）. Flow cytometry（FCM） was used to measure the ROS level of NRCMs, and the activity of Caspase 3 and Caspase 9 were evaluated with ELISA. Results： Propofol significantly inhibited the ROS production （P 〈 0.05） and the activity of Caspase 3 and 9 （P 〈 0.01） induced by H2O2（P 〈 0.01） in neonatal rat cardiomyocytes. Conclusion： Propofol inhibited the ROS production and the activity of caspase 3 and caspase 9 of mitochondria, which may contribute to the protective effect of propofol on cardiomyocytes.