人参多糖对NK92-MI细胞杀伤活性的影响及机制

Effects of ginseng polysaccharides(GPS) on the cytotoxicity of the NK92-MI cells

目的:研究人参多糖对NK92-MI杀伤活性的影响及机制。方法:采用200mg/L或400mg/L GPS作用于NK92-MI细胞,24h后收集细胞,采用CCK-8试剂盒检测GPS对NK92-MI细胞杀伤活性和增殖活性的影响;采用流式细胞术检测GPS对NK92-MI细胞活化受体(NKp30、NKp44、NKp46、NKG2D)表达的影响;采用western blot技术检测NK细胞杀伤介质(穿孔素和颗粒酶B)表达水平。结果:与正常对照组相比,GPS可明显促进NK细胞杀伤活性,上调活化受体(NKp30、NKp44、NKp46)、杀伤介质(穿孔素和颗粒酶B)的表达水平(P<0.05)。结论:GPS通过上调活化受体(NKp30、NKp44、NKp46)的表达促进NK92-MI细胞的活化,并且通过上调杀伤介质(穿孔素和颗粒酶B)的表达提高NK92-MI细胞的杀伤活性。

Objective To research the influence and mechanism of Ginseng polysaccharides （GPS） on the cytotoxicity of the NK92 - MI cells. Methods CCK8 kit was adopted to detect the cytotoxicity of NK92 - MI cells, Flow cytometric analysis was adopted to detect the expression of activated receptors, western blot was adopted to detect the expression level of perfofin and Granzyme B. Results Compare to the normal control group, GPS could increase the cytotoxicity, expression of NKp30, NKp44, NKp46, perforin and granzymeB. Conclusion GPS can increase the cytotoxieity of NK92 - MI cells by increasing expression of activated receptors, perforin and granzyme B.