CEP55在人胶质瘤组织的表达及对人胶质瘤U251细胞增殖和凋亡的影响

Expression of CEP55 in human glioma and its effect on the proliferation and apoptosis of glioma cell line U251

目的检测中心体相关蛋白55(CEP55)在人胶质瘤组织中的mRNA和蛋白表达水平;抑制人胶质瘤U251细胞中CEP55表达,观察其对U251细胞增殖、凋亡的影响。方法实时荧光定量聚合酶连锁反应(q-PCR)、蛋白质印记法(Western Blot)检测40例不同级别人胶质瘤组织和10例正常脑组织中CEP55的表达;RNAi抑制U251胶质瘤细胞CEP55基因表达,采用细胞增殖毒性检测试剂盒(CCK-8)和胸腺嘧啶核苷类似物(edu)检测CEP55对U251细胞增殖的影响,采用流式细胞仪分析CEP55对细胞凋亡的影响。结果 CEP55在40例人胶质瘤组织中表达显著高于正常脑组织(P<0.01),但高级别胶质瘤CEP55的表达与低级别胶质瘤没有显著差异(P>0.05)。在RNAi抑制U251细胞CEP55表达后,细胞的增殖显著受抑(P<0.01),细胞的凋亡显著增加(P<0.01)。结论人胶质瘤组织细胞较正常脑组织CEP55表达明显增高,抑制CEP55的表达抑制人胶质瘤U251细胞的增殖,同时促进人胶质瘤细胞的凋亡。

Objective The expression of centrosomal protein 55 （ CEP55 ） in human glioma cells was detected and the effect of CEP55 on proliferation and apoptosis of human glioma cell line U251 cells was analyzed. Methods The expression of CEP55 was measured by quantitative real-time PCR and Western Blot in 40 human glioma tissues of variable grades and 10 normal brain tissues. The proliferation of glioma cell line U251 was measured by CCK-8 kits and edu kits and the apoptosis of glioma cell line U251 was measured by flow cytometry after CEP55 silencing. Results The expression of CEP55 was increased significantly in 40 glioma tissues compared to 10 normal brain tissues （ P 〈 0.01 ）. However, there was no statistical difference in the expression of CEP55 between patients with high grade glioma and lower grade glioma （ P 〉 0.05 ）. The proliferation of U251 cells was inhibited markedly after CEP55 knockdown by RNAi （P 〈0.01 ） ; the apoptosis of U251 ceils was profoundly increased after CEP55 knockdown （ P 〈 0.01 ）. Conclusion The expression of CEP55 is significantly increased in human glioma cells compared with normal brain tissue; the overexpression of CEP55 promotes the proliferation of glioma U251 cells and inhibits the apoptosis of U251 cells.