5-ALA-PDT对A431细胞的抑制作用及细胞凋亡的诱导作用

Effects of 5-ALA-PDT on proliferation and apoptosis of A431 cells

目的探讨5-ALA-PDT对A431细胞增殖与凋亡的影响。方法将细胞分为对照组以及不同剂量的5-ALA-PDT处理组(5-ALA孵育浓度分别为:0.05、0.1、0.2、0.4、0.8 mg/ml,激光能量密度分别为1.25、2.5、5 J/cm^2),处理24 h后采用MTT法检测5-ALA-PDT对A431细胞存活率的影响,采用流式细胞术(FCM)检测5-ALA-PDT对A431细胞的周期及凋亡的影响。结果 MTT法检测结果显示在不同的5-ALA孵育浓度(0.05、0.1、0.2、0.4、0.8 mg/ml)和不同的激光能量密度(1.25、2.5、5 J/cm^2)处理A431细胞时,当激光的能量密度和孵育的浓度都较低的时候.在5-ALA-PDT的作用下,A431细胞的杀伤曲线平缓的下降,然而伴随着激光的能量密度和光敏剂5-ALA浓度的不断加强,可见抑制曲线的下降速度十分迅速,各组细胞的存活率在达到较高的强度时呈现相接近的趋势。当5-ALA孵育浓度达到0.8 mg/ml时,不同激光能量密度处理的细胞存活率差异无统计学意义(P>0.05);5-ALA-PDT作用后使A431细胞滞留在G0/Gl期,A431细胞的增殖指数(PI)降低;与对照组相比,5-ALA-PDT处理后可以显著增加A431细胞的凋亡率和坏死率(P<0.05)。结论 5-ALA-PDT显著抑制皮肤鳞状细胞癌A431细胞的增殖,促进A431细胞的凋亡。

Objective To analyze the effect of 5-ALA PDT on the proliferation and apoptosis of the squamous cell carcinoma A431.Methods Cells were divided into control group and treatment group at different doses of 5-ALA-PDT （5-ALA incubation concentration was 0.05, 0.1, 0.2, 0.4, 0.8 mg/ml respectively, and the laser energy density was 1.25, 2.5, 5 J/cm^2 respectively）. After 24 h treatment, MTT method was used to test the effect of 5-ALA-PDT on the survival of A431 cells, while the flow cytometry method （FCM） was used to test the effect of 5-ALA-PDT on A431 cells＆#39; cycle and apoptosis.ResultsThe result of MTT method showed that when A431 cells were treated by 5-ALA of different incubation concentrations （0.05, 0.1, 0.05, 0.1, 0.05 mg/ml） and of different laser energy densities （1.25, 2.5, 5 J/cm^2）, the killing effect of A431 cells by 5-ALA-PDT was positively related to the incubation concentration and laser energy density. But when the incubation concentration of 5-ALA reached 0.8 mg/ml, there was no obvious difference （P＆gt;0.05） in the survival rate of cells treated at different laser energy densities. The flow cytometry method （FCM） test results showed that after 5-ALA-PDT treatment, A431 cells were stranded in the G0/ Gl period, while the proliferation index （PI） of A431 cells was reduced. The Annexin V-FITC-PI double dyeing results showed that compared with the control group, the apoptosis and necrosis rate of A431 increased significantly （P〈0.05） after 5-ALA-PDT treatment.Conclusion5-ALA-PDT treatment can inhibit the proliferation of skin squamous cell carcinoma A431 cells, but promote the apoptosis of A431 cells.