IL-23对强直性脊柱炎的诊断意义及其受体表达的研究

Significance of IL-23 on the diagnosis of ankylosing spondylitis and its receptor expression

目的:研究白细胞介素23(IL-23)对强直性脊柱炎(AS)患者的临床诊断意义及其受体表达情况。方法:选择2012年11月至2013年11月丹江口市妇幼保健院收治的AS患者40例为AS组,另外选择同期健康体检者40例作为健康对照组。测定两组受试者血清IL-23、红细胞沉降率(ESR)、C反应蛋白(CRP)和免疫球蛋白(IgA)水平;收集分离两组受试者外周血中单个核细胞(PBMCs)并行体外细胞培养,收集细胞,利用Western blotting技术检测IL-23 p19R蛋白表达变化。结果:AS患者血清中IL-23水平显著高于健康对照组[(630±69)ng·L^(-1)vs(84±7)ng·L^(-1),P<0.05],且AS组血清IL-23水平与Bath AS疾病活动指数(BASDAI)、ESR和CRP的浓度呈正相关(r值分别为0.598、0.602、0.548、0.436,P值分别为0.004、0.005、0.040、0.030)。AS组PBMCs经IL-23刺激后,其IL-23 p19R蛋白表达显著升高。结论:AS患者血清中IL-23水平与BASDAI、ESR、CRP及IgA的水平变化呈正相关,IL-23可能通过改变IL-23 p19R蛋白的变化来介导CD_4^+T向Th17分化,参与AS的发病。

Objective： To study the significance of IL-23 on the diagnosis of ankylosing spondylitis （AS） and its receptor expression. Methods： The serum level of IL-23 in AS patients and healthy controls, erythrocyte sedimentation rate （ ESR）, C- reactive protein （CRP） and immunoglobulin （IgA） were detected ; the peripheral blood mononuclear cells（PBMCs） were separated and collected in the AS patients and healthy controls, the cells were then cultured in vitro,IL-23 pl9R protein expression was assessed using Western blotting technique. Results： The concentration of serum IL-23 in AS patients significantly increased compared with healthy controls group with a significant difference [ （630 ± 69） ng·L^-1 vs （ 84 ± 7 ）ng·L^-1 , P 〈 0.05 ], and the concentration of serum IL- 23 with BASDAI index,ESR and CRP concentrations were positively correlated （ r value were 0. 598,0. 602,0. 548, 0. 436, P values were 0. 004,0. 005,0. 040,0. 030, respectively）. The AS group of PBMCs after IL-23 stimulation, the IL-23 pl9R protein expression increased significantly, compared with healthy controls with a significant difference（P 〈 0. 001 ）. Conclusion： The concentration of serum IL-23 in AS patients is positively correlated with BASDAI, ESR, CRP, IgA via changing 23 pl9R protein expression and mediating CD4^＋ T into Thl7 differentiation, thus influencing the pathogenesis of AS.