高压脉冲电场结合炭黑复合涂层对硅藻活性的影响研究

The Effect of High Voltage Pulse Electric Field on Inactivation of Diatom Attached to Carbon Black Composite Epoxy Coating in Marine Environment

随着环境管制条例增多以及社会对传统有毒防污涂料安全问题的关注,研究环保海洋生物防污方法迫在眉睫。采用TTC-脱氢酶活性测试来表征高压脉冲电场(HPEF)结合炭黑环氧防腐复合涂层的防污性能。采用SEM观察分析炭黑复合涂层上的硅藻经脉冲电场处理前后的微观结构和形貌特征。经HPEF处理后舟形藻和海链藻细胞出现凹陷、破裂及有机质溢出,说明HPEF杀藻可采用"电穿孔"理论解释。在通电时间10 min、频率为23.15 k Hz、占空比为0.5、电压为19 k V条件下,硅藻的失活率达到最大。在炭黑复合涂层上的海链藻最大失活率为89.2%,舟形藻最大失活率为92.1%。适量的炭黑可以增强环氧树脂的力学性能。炭黑复合涂层的力学性能取决于炭黑的添加量,当炭黑添加量为0.3%时可以得到综合力学性能较好的复合涂层。

With increasing restrictions of environmental regulations and safety concerns over traditional poisonous coatings, it is highly desirable to develop a new green method for anti-biofouling in marine environment. In this paper, a green method that is high voltage pulse electric field （HPEF） combined with carbon black composite coating was introduced to inhibit the adhesion of diatom. Results are presented from diatom inactivation assays on the composite coating upon applications of HPEF. Diatom on the composite coating surfaces was visualized using SEM for exploring the mechanism of why HPEF inhibit the adhesion of diatom. Using pulses of 10 rain in duration, 23.15 kHz in frequency, 0. 5 in duty cycle and 19 kV in pulse amplitude, diatom inactivation rate reached the maximum. The largest inactivation rate of thalassiosira was 89. 2%. The maximum inactivation rate of navicular was 92. 1%. Furthermore, we find that carbon black could increase the mechanical properties of epoxy resin, and the optimum content of carbon black was 0. 3%.