白三叶化感作用相关基因CHS的克隆及生物信息学分析

Cloning and Bioinformatics Analysis of White Clover Allelopathy Related Genes CHS

白三叶(Trifolum repens)化感物质中含有多种化学成份,主要含有酚类与萜类及黄酮类物质,而查尔酮合成酶(CHS)是类黄酮途径的第一限速步骤,对于黄酮类物质的合成至关重要。本试验从白三叶中克隆了CHS基因cDNA序列,并对白三叶CHS基因进行了生物信息学分析。结果表明克隆到的CHS基因全长cDNA序列1473bp,其中包含了1170bp的开放阅读框(ORF),起始密码子为ATG,终止密码子为TAA,103bp的5′UTR和200bp 3′UTR编码389氨基酸。且白三叶的CHS基因与同属于豆科的苜蓿CHS、大豆CHS、鹰嘴豆CHS和豌豆CHS亲缘关系最近,处于同一分支,这与植物分类相一致。

There was a variety of chemical compositions in allelochemicals of White clover（Trifolium repens L. ）, which mainly contains phenols, terpenoids and flavonoids, while chalcone synthase （CHS） is the first rate-limiting step of the flavonoid pathway which is essential for flavonoids synthesis. In this study, the full-length CHS gene in white clover was cloned and was analyzed in bioinformatics. The results showed that the cloned full-length cDNA sequence of CHS gene was 1473 bp, which contains an open reading frame （ORF） of 1170 bp（the start codon was ATG, termination codon was TAA）, 103 bp 5＇UTR and 200 bp 3＂UTR. CHS gene in white clover and that in other legume plant alfalfa, soybean, chickpeas and peas had closest relationship and was in the same branch, which is consistent with plant classification.