定心藤总黄酮对动脉粥样硬化大鼠血脂及一氧化氮和血管内皮生长因子的影响

Effects of Mappianthus Iodoies Flavonoids on Blood Lipid,NO and VEGF in Atherosclerosis Rats

目的探讨定心藤总黄酮对动脉粥样硬化(As)大鼠血脂及一氧化氮(NO)和血管内皮生长因子(VEGF)的影响。方法 SD雄性大鼠60只,随机分为6组:正常对照组(NC)、模型对照组(MC)、阳性对照组(PC,辛伐他汀组)、定心藤总黄酮低剂量组(MIF1)、定心藤总黄酮中剂量组(MIF2)、定心藤总黄酮高剂量组(MIF3)。建立As大鼠模型,造模同时给予药物干预,6周后,一侧眼球摘除法取血,测定血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C);继续喂养12周后,心脏采血,测定超氧化物歧化酶(SOD)、丙二醛(MDA)、总氧化能力(T-AOC)、NO和VEGF的水平,分离胸主动脉,制备病理切片。结果与NC组相比,MC组TC、TG、LDL-C、AI、LDL-C/HDL-C、MDA、VEGF显著升高,SOD、T-AOC显著降低,差异有统计学意义(P<0.05);与MC组相比,MIF组可以降低As大鼠血清TC、TG、LDL-C、MDA和升高HDL-C、SOD、T-AOC的水平,可以改善As相关因子NO的浓度,差异有统计学意义(P<0.05),高剂量的MIF可以改善As相关因子VEGF的浓度,差异有统计学意义(P<0.05)。结论 MIF可能通过提高机体的抗氧化能力调节NO和VEGF的释放,达到降血脂及预防As形成的作用。

Objective To discuss the influence on blood lipid, NO and VEGF of mappianthus iodoies flavonoids （MIF） in atherosclerosis （As） rats. Methods Sixty male SD rats were random allocated. As rats model was estab- lished, while drug intervention were also given. Six weeks after the treatment, blood samples were collected using eyeball removal method. Serum total cholesterol （TC）, triglycerides （TG）, low- density lipoprotein cholesterol （LDL- C）, and high density lipoprotein cholesterol （HDL- C） were assessed. After 12 weeks, culling heart blood samples were collected, as superoxide dismutase （ SOD）, malondialdehyde （MDA） , total oxidation ability （ T - AOC ） , nitric oxide （ NO ） and vascular endothelial growth factor （VEGF） were assessed. The thoracic aortas were collected for pathological observa- tion. Results Compared with NC group, TC, TG, LDL - C, AI and LDL - C/HDL - C were significantly increased and MDA, VEGF, SOD and T - AOC were significantly reduced in the MC group （P 〈 0. 05）. Compared with MC group, MIF can significantly reduce the serum TC, TG, LDL - C, SOD, and MDA ; increased HDL - C and T - AOC ; and improved the As related factor NO （ P 〈 0. 05 ）. High dose MIF significantly improved VEGF concentration （ P 〈0. 05 ）. Conclusion MIF may increase the antioxidant capacity, and adjust the release of NO and VEGF factor, thus to prevent atherosclerosis.