采用在体单向肠灌流模型研究荷叶多成分整体的肠渗透性

Overall intestinal permeability of multiple components in lotus leaf by in situ single pass intestinal perfusion models

研究荷叶多成分整体的肠渗透性,明确荷叶成分间吸收过程中的相互作用。采用大鼠在体单向灌流法。结果表明,荷叶中荷叶碱、去甲基荷叶碱、芦丁、异槲皮苷、紫云英苷、槲皮素、山柰酚7种单体成分的有效渗透系数(Peff)大于0.5×10^(-4)cm·s^(-1),在生物药剂学分类系统(biopharmaceutics classification system,BCS)肠渗透属性中,属高渗透性成分,而儿茶素、金丝桃苷为低渗透性成分。然而,在荷叶总提取物多成分环境下,成分的渗透行为发生了变化,对荷叶中不明确成分进行半定量分析发现,在多成分环境下,可标识的9个成分中有3个成分Peff小于0.5×10^(-4)cm·s^(-1)。在多成分环境下,7个未知成分中,3个成分属于高渗透性成分,4个成分属于低渗透性成分。该研究初步明确了荷叶中多成分整体的肠渗透性,为揭示中药多成分环境下的吸收机制奠定一定的基础。

To investigate the overall intestinal permeability of multiple components in lotus leaves and make clear the interaction in composition absorption process.Rat single-pass intestinal perfusion technique was used, and the results showed that the Peff values of nuciferine, demethylanuciferine, rutin, quercetin, kaempferol from lotus leaf were greater than 0.5＆#215;10-4 cm＆#183;s-1.In the biopharmaceutics classification system （BCS） intestinal permeability property, these ingredients were high permeable components, while the hyperin was low permeable component.However, in the multi-component environment of the lotus leaf extract, component permeation was changed.Semi quantitative analysis of the unclear components showed that under the multi-component environment, four in seven components with relatively high contents had a Peff value less than 0.5＆#215;10-4 cm＆#183;s-1, indicating these 4 components were of low permeability, while other 3 components were of high permeability.The results could be valuable to make clear the overall intestinal permeability of multiple components in lotus leaf, and lay a foundation for studying the mechanism of the lipid-lowering effect of lotus leaf.