封闭肠环法用于葛根多成分肠壁吸收代谢研究

Intestinal absorption and metabolism of Puerariae Lobatae Radix decoction by in situ closed-loop method

中药生物药剂学分类系统在药物溶解性和渗透性研究的基础上,强调中药的多成分环境特点。该文采用封闭肠环法,同时结合液质联用技术对葛根水提液多成分肠吸收代谢情况加以研究分析,为中药多成分的生物药剂学分类系统渗透性评价方法提供遴选依据。结果在葛根水提液中推测并鉴别出36个成分,其中17个成分可在血浆样品中检测到,表明其可以吸收入血,可初步作为中药生物药剂学分类系统中肠渗透性评价成分;另外有19个成分未在血浆样品中检测到,表明其可能未吸收或与肠壁酶结合、代谢。

Biopharmaceutics classification system of Chinese materia medica （CMMBCS） emphasizes characteristic of the multi-component environment based on the drug solubility and permeability.In this study, the in situ closed-loop method combined with LC-MS technique was utilized to study the intestinal absorption and metabolism of Puerariae Lobatae Radix decoction （PLRD）, providing selection basis for intestinal permeability components in CMMBCS.A total of 36 components were identified from PLRD.Among them, 17 components could be detected in the plasma sample, indicating that 17 components could be absorbed into blood, so these 17 components could be used as intestinal permeability evaluation components in CMMBCS.The other 19 components were not detected in the plasma sample, suggesting that they may not be absorbed or metabolized by the gut wall enzymes.