离体法及在体法对黄连水煎液中肠吸收多成分的初步研究

In vitro and in vivo intestinal absorption of Huanglian decoction in multi-component environment

药物药效的发挥与其在机体内始终处在吸收、分布、代谢、排泄这种动态变化的过程密不可分,而吸收作为口服药物进入机体的首要环节格外受到重视。中药作为多成分与多靶点的药物,单一成分的性质与其在多成分环境中所表现出来的性质会有差别。该文以黄连提取物中的生物碱为主要研究对象,建立黄连中生物碱成分含量测定的分析方法。并通过离体翻转肠囊实验及肠道灌流并行采血实验初步锁定黄连水提液中可透过肠壁吸收并吸收入血的成分,为后续对黄连多成分的吸收代谢研究提供数据参考与支持。

The effect of drugs in the body is always inseparable with the dynamic processes of absorption, distribution, metabolism and excretion.For oral drug, absorption, as the first link to enter the body, is particularly valued.Traditional Chinese medicine has multiple components and multiple targets, and the nature of its single composition is different from that in the multicomponent environment.Alkaloids in Huanglian extract was used as the main object in this study to establish an analytical method for determining the content of alkaloids in Huanglian.In addition, the compositions of the Huanglian aqueous extract solution which can be absorbed through intestinal wall into blood, were initially determined by the means of everted gut sac and in intestinal perfusion with venous sampling experiment.This paper can provide data reference and support for the further study on the absorption and metabolism of Huanglian.