高效液相色谱法测定复方枇杷喷托维林颗粒中枸橼酸喷托维林的含量

Simultaneous determinationof pentoryverine citrate in Compound Loquat and Pentoryverine Granules by HPLC

目的建立复方枇杷喷托维林颗粒中枸橼酸喷托维林含量的高效液相测定方法。方法采用Aglient C18(150 mm×4.6 mm,5μm)色谱柱;流动相:1%三乙胺溶液(用磷酸调p H至3.0)-乙腈(70∶30);流速:1.0 m L·min^(-1);检测波长:210nm。结果枸橼酸喷托维林在0.02~1.01 g·L^(-1)的质量浓度范围内线性关系良好,Y=6 652 832.11 X-8 399.71(r=0.9999),平均加样回收率为99.4%,RSD=1.2%。结论该法简便、准确,可用于复方枇杷喷托维林颗粒中枸橼酸喷托维林的质量控制。

Objective To e s tabl ish a HPLC method for the dete rmina tion of pentoryverine ci tra te in Compound Loquat and Pentoryver-ine Granules. Methods The chromatorgraphic condi tions were as follows ： Agl ient C18 （ 150 mm x 4 . 6 m m ,5 jjim） column was used. 1 % triethylamine（ pH 3.0） -acetonitrile（70- 30） was used as mobile phase,and the flow rate was at 1.0 mL ？ min 1. The detect wave-length was at 210 nm,the column temperature was 35 . Results T he ca libra tion curves were line ar ly in th e range of 0 . 0 2 -1 .0 1 g ？L_1, the regression equation was F = 6 652 832. 11 Z - 8 399. 71 （ r = 0. 999 9） . The average recover was 9 9 . 4% . Conclusion The method was simple, accurateand can be used for quality control.