基于线粒体COⅠ基因部分序列的缅甸安小叶蝉地理种群遗传多样性研究

Analysis of Genetic Diversity among Different Geographic Populations of Anaka burmensis (Hemiptera:Cicadellidae) Based on Part of mt DNA COⅠGene Sequences

缅甸安小叶蝉Anaka burmensis是一种取食竹子的害虫,为了探讨该物种不同地理种群的遗传多样性,本研究首次基于线粒体COⅠ基因部分序列对我国26个地理种群共241个样本进行了研究,选取长度为615 bp的基因序列,并运用DNASP、MEGA等分析得出,该片段中有546个保守位点、69个变异位点和33个单倍型。种群的单倍型多样性指数为0.845,核苷酸多样性指数为0.008 77,基因流为0.598 5,种群间的固定系数为0.729 15,表明种群间遗传多样性水平高、遗传分化大、基因交流水平较低。中性检验Tajima's D为-1.658 98,0.10>P>0.05,Fu's Fs值为-5.787,P>0.10。分子变异结果显示,该物种遗传变异主要来自种群间,变异百分率为72.92%,而种群内的遗传变异低,仅为27.08%。研究结果得出该物种遗传结构,可为今后从事叶蝉类昆虫的分子生物学研究及该虫的防治提供理论基础资料。

Anaka burmensis is an important pest which feed on bamboo. To investigate the genetic variation of A. burmen-sis among different geographic populations in China, the 615 bp segments of the mitochondrial cytochrome coxidase subunit I （CO I） gene of A. burmensis individuals from 26 geographic populations were analyzed by using DNASP, MEGA, etc.The results showed that there were 546 conserved sites, 69 mutation sites, 33 haplotypes in the fragments; a high level of genetic diversity （ haplotype diversity index ： 0. 845 ; nucleotide diversity index ： 0. 008 77 ） in the total population was de-tected. Additionally, high genetic differentiation （fixation index： 0.729 15） was found among different geographic popula-tions but with low gene flow level （0. 598 5） . The differences of the Neutral test （Tajima＇s D = -1 . 658 98, 0. 10 〉 P 〉0. 05, Fu’s = - 5. 787, P 〉 0. 10） were not significant. The result of molecular variance analysis showed that the genetic differentiation among populations was 72. 92% and higher than that of within populations （27. 08% ） . Therefore, here we concluded the genetic structure of A. burmensis can provide a theoretical basis for future research of molecular biology and the control of this insect.