β-西柏三烯二醇对拟南芥的化感效应及其作用机理研究

Allelopathic Effect and Mechanism of β-cembrenediol on the Growth of Arabidopsis thaliana

以拟南芥幼苗为受体植物,研究了不同浓度二萜类化合物β-西柏三烯二醇对拟南芥生长的活性作用以及作用机理,以揭示萜类化感物质的作用机制。结果表明:(1)β-西柏三烯二醇对拟南芥幼苗的鲜重具有显著抑制作用,当处理浓度为50μmol/L时抑制作用达到显著水平,并对拟南芥鲜重的抑制率均大于30%。(2)经过β-西柏三烯二醇处理后,拟南芥叶片中叶绿素a、b含量显著下降,且抑制作用随处理浓度的增大和处理时间的延长而增加。(3)随着β-西柏三烯二醇处理浓度的增大,拟南芥中过氧化物酶(POD)和超氧化物歧化酶(SOD)的活性逐渐显著降低,而过氧化产物MDA含量显著增加。可见,β-西柏三烯二醇通过抑制拟南芥体内抗氧化酶的活性,引起膜脂过氧化,从而抑制拟南芥幼苗的正常生长和生理过程,且这种抑制作用随浓度的升高而增强。

In this study,we investigated the allelopathic effects of β-cembrenediol and its phytotoxic mechanism on Arabidopsis thaliana seedlings.The results showed that：（1）β-cembrenediol significantly reduced fresh weight of A.thaliana.The fresh weight of A.thalianaseedlings treated with the compound at high concentrations（50μmol/L）was reduced more than 30% compared to the control;（2）After β-cembrenediol treatment,the levels of chl a,b in A.thalianaleaves both declined in a concentration-dependent and time-dependent manner;（3） β-cembrenediol inhibited the activities of POD and SOD,while increased the content of MDA.These results indicated that under the stress of β-cembrenediol,the activities of enzyme were inhibited and the degree of membrane lipid peroxidation was strengthened,and chlorophyll content was decreased,ultimately resulted in growth inhibition of A.thalianaseedlings.