不同剂量分割模式照射Eca109细胞TNF和SODD的表达

Expression of TNF and SODD in Eca109 cells under different dose fractionation mode irradiation

目的:通过模拟不同剂量分割方式照射食管癌细胞Eca109,观察比较各组别凋亡相关基因肿瘤坏死因子(tumor necrosis factor,TNF)和死亡结构域沉默子(silencer of death domains,SODD)的各组别表达差异并分析原因及意义,尝试为临床实施的非常规放疗提供潜在基础理论依据。方法:设计具有相同BED的4种剂量分割模式,照射处于指数生长期的Eca109细胞后,RT-PCR检测各组TNF mRNA和SODD mRNA表达变化,Western Blot检测各组TNF蛋白和SODD蛋白表达变化。结果:各组TNF mRNA及蛋白的表达量均数差值比较,有显著性差异;各组SODD mRNA及蛋白的表达均数差值比较,有统计学意义。结论:模拟照射各组中超分割组TNF mRNA表达上调幅度最大,SODD mRNA上调幅度亦最大。提示在射线诱导肿瘤细胞以某种方式走向死亡过程中,存在着促凋亡基因和抗凋亡基因之间的博弈。模拟照射各组中TNF蛋白和SODD蛋白均大部分下调,提示除DNA是射线的主要损伤靶点外,射线对其他细胞器的损伤作用亦不容忽视,因为它们的功能状态可能影响到蛋白质的合成。

ObjectiveHope to provide some theoretical basis for unconventional radiotherapy, we observe and compare expressionof apoptosis related genes （ TNF and SODD ） between groups by simulating different dose fractionation on esophageal cancer cellsEcal09. Methods：Design the dose fractionation mode with the same BED. Irradiation is exponential growth phase Ecal09 of cells. DetectTNF mRNA and SODD mRNA, expression in each group with RT-PCR on the first day after simulation irradiation. Detect TNF proteinand SODD proteins, expression in each group with Western Blot on the first day after simulation irradiation. Results： Mean differencesof the TNF mRNA , s and protein , s expression quantity in each group were compared, all were statistically significant; Meandifferences of the SODD mRNA, s and protein , s expression quantity in each group were com pared, all were statistically significant.Conclusion： Among the simulation irradiation groups,the hyperfractionated group, s TNF mRNA , s expression was the highest, so wasSODD mRNA. It suggests that a fight should exist between promoting apoptotic genes and anti-apoptotic gene in the process of inducedtumor cells , dying. In the analog irradiation groups, both TNF protein and SODD protein suffered massive reduction. It suggests that injuryof other organelles except DNA can not be ignored, because their functional status may affect the synthesis of protein.