山仙颗粒对S-180荷瘤小鼠T细胞活性及瘤组织中Caspase-9影响的实验研究

The effect of Shanxian Granule on the activity of T cell and Caspase-9 in S-180 tumor-bearing mice

目的:通过建立昆明小鼠S-180荷瘤模型,观察山仙颗粒(SXG)对S-180荷瘤小鼠T细胞活性及肿瘤组织中Caspase-9表达的影响,探讨其抗肿瘤的分子机制。方法:构建S-180荷瘤小鼠模型,并随机分为5组,即空白对照组、模型组、SXG低、中、高剂量组,早晚灌胃给药,连续30天。颈椎脱臼处死小鼠,游离脾脏,剥离肿瘤组织。应用MTT比色法及免疫组织化学法分别检测荷瘤小鼠脾脏T细胞活性与S-180实体瘤组织中Caspase-9的表达。结果:SXG用药各组小鼠T细胞对于S-180细胞的抑制率均高于模型组,与模型组比较均有显著差异(P<0.01);SXG用药各组,S-180肿瘤组织中Caspase-9的表达明显上调,与模型组相比较具有显著差异(P<0.01)。结论:SXG有抑制肿瘤的作用,其机制与促进Caspase-9介导的肿瘤细胞凋亡密切相关。

Objective： To investigated the effects of Shanxian Granule on the activity of T-cell and Caspase-9 in S-180 tumor-bearing mice,and elucidated the probable molecular mechanism of antitumor. Methods： The S-180tumor-burdened model mice were randomly divided into five groups： Control group,model group and the different dosage of SXG group gavage administration in each morning and evening for 30 consecutive days. The mice were sacrificed by cervical dislocation. Then,the spleen was dissected and the tumor tissues were dissected. The activity of T cell and Caspase-9 were respectively detected in the spleen and solid tumor of S-180 tumor-burdened model mice by MTT colorimetry and immunohistochemistry. Results： The inhibition rate of T cells on S-180 cells in SXG drug groups was higher than that in model groups（ P 〈0. 01）. The expression of Caspase-9 also obviously up-regulated in tumor tissue of SXG administration groups compared with those of the model group（ P 〈0. 01）. Conclusion： SXG can inhibit the tumor growth by the mechanism of promoting Caspase-9-mediated apoptosis of tumor cells.