miRNA302b在子宫内膜癌中的表达及其与临床病理参数的关系

The expression of miRNA302b in endometrial cancer and its relationship with clinical pathological factors

目的:检测miRNA302b在子宫内膜癌组织中的表达,并探讨其表达与临床病理参数之间的关系。方法:利用Real-time PCR方法检测40例子宫内膜癌组织及40例正常子宫内膜组织中miRNA302b的相对表达量,并分析miRNA302b在不同临床病理参数的子宫内膜癌标本中表达的差异性。结果:子宫内膜癌中miRNA302b相对表达量(1.195 5±1.645 91)低于其在正常子宫内膜组织中的相对表达量(2.484 6±2.860 33),其差异具有统计学意义(P=0.016<0.05);无淋巴结转移子宫内膜癌组中miRNA302b相对表达量(0.798 2±1.095 60)低于有淋巴结转移组(3.447 4±2.459 57),其差异具有统计学意义(P=0.046<0.05);临床分期为I期的子宫内膜癌组织中miRNA302b的相对表达量(0.483 5±0.438 12)低于其在II、III、IV期的子宫内膜癌组织中的相对表达量(3.331 7±2.088 17),其差异有统计学意义(P=0.002<0.05);组织分化程度为高分化组miRNA302b相对表达量(0.676 4±0.776 56)低于其在中低分化组的相对表达量(2.159 6±2.329 68),其差异有统计学意义(P=0.036<0.05)。但miRNA302b在子宫内膜癌组织中的表达与浸润深度、远处转移等临床病理参数无明显统计学关系(P>0.05)。结论:子宫内膜癌组织中miRNA302b表达低于其在正常子宫内膜组织中的表达,且在不同临床分期、组织学分化程度及有无淋巴转移的子宫内膜癌组织中,miRNA302b的表达存在统计学差异,提示其可能在子宫内膜癌发生、发展中发挥作用。

Objective： To detect the expression of miRNA302 b gene in tissue of endometrial cancer,and to explore its relationship with different clinical pathological factors. Methods： Quantify miRNA302 b expression in 40 endometrial cancer tissues and the same number of normal endometrial tissues with quantitative real-time PCR. Then,evaluate the association of miRNA302 b expression with the clinicopathologic features. Results： The expression of miRNA302 b was significantly decreased in endometrial tissues samples compared to normal tissues（ P〈 0. 05）. The expressions of the miRNA302 b were（ 0. 483 5 ± 0. 438 12）,（ 3. 331 7 ± 2. 088 17） in stage I and II-IV endometrial cancer,and the difference was statistically significant（ P〈 0. 05）. The expressions of the miRNA302 b were（ 3. 447 4 ± 2. 459 57）,（ 0. 798 2 ± 1. 095 60） in endometrial cancer tissue group with or without lymph node metastasis,and the difference was statistically significant（ P〈 0. 05）. Also,the expression of miRNA302 b in patients with different differentiation degrees was statistically significant（ P 〈0. 05）. However,miRNA302 b expression was not significantly correlated with the other clinical pathological factors. Conclusion： The expression of miRNA302 b was lower in endometrial cancer tissue compared to normal endometrial tissue,and the expression level of miRNA302 b was statistically different in different surgical-pathological stages,different differentiation degrees,with or without lymph node metastasis. The abnomal low expression of miRNA302 b may be associated with carcinogenesis and development of endometrial cancer.