摘要
羊〔Colocasia esculenta（Linn.）Schott〕通常用球茎繁殖,其种质保存方式主要为建立种质圃,但具有占用空间大、投入较高、易遭受胁迫等问题.超低温保存技术具有稳定、经济、有效等优点,适合无性繁殖和非正常性种子繁殖植物的种质保存,而玻璃化法作为-种更优的保存技术已成功应用于多种植物的种质保存.
In order to screen suitable vitrification conservation conditions for shoot tip of Colocasia esculenta‘Hongxiangyu’,pre-culture,vitrification protection and thawing conditions were compared. The results show that with increasing of sucrose concentration in the medium and extending of pre-culture time,survival rate of shoot tip increases gradually. After different loaded and dehydrated measures,water content in shoot tip is lower than that of the control,while survival rate of shoot tip increases significantly than that of the control. Thawing for 1-2 min at 40 ℃-60 ℃ generally has no significant effect on survival rate of shoot tip. Suitable cryopreservation steps of vitrification as follows: shoot tip precultured for 3 d in medium with 0. 6 mol·L^(-1) sucrose firstly,then loaded for 30 min in 60% PVS2,dehydrated for 15 min in PVS2,put in PVS2 and conserved in liquid nitrogen,and shoot tip thawed for 1-2 min in 40 ℃.
出处
《植物资源与环境学报》
CAS
CSCD
北大核心
2017年第1期116-118,共3页
Journal of Plant Resources and Environment
基金
江苏高校优势学科建设工程资助项目(PAPD)
关键词
红香芋
茎尖
玻璃化保存
存活率
Colocasia esculenta ‘Hongxiangyu’
shoot tip
vitrification conservation
survival rate
