黄腐酚诱导非小细胞肺癌细胞凋亡的初步研究

Apoptosis of Non-small Cell Lung Cancer Induced by Xanthohumol

目的探索黄腐酚对人非小细胞肺癌细胞株A549与H1650凋亡的影响。方法采用CCK-8法检测黄腐酚对A549与H1650细胞增殖的影响;流式细胞术分析黄腐酚对两种细胞凋亡的影响;Western Blot法检测两种细胞被不同浓度黄腐酚干预后细胞中凋亡相关蛋白Bax、Bcl-xl、pro-caspase-3的表达。结果黄腐酚能有效抑制A549与H1650细胞的增殖,其抑制强度与浓度呈正相关,对H1650细胞有更好的抑制作用;两种细胞的凋亡率随黄腐酚浓度的增大而上升,5μmol·L-1诱发的凋亡率与同浓度顺铂无差异(P>0.05);两种细胞中凋亡相关蛋白的表达与黄腐酚浓度相关。结论黄腐酚可能通过调控Bcl-xl、Bax、Caspase-3及其相关基因使非小细胞肺癌细胞发生凋亡。

Objective To investgate the effect of xanthohumol on apoptosis of human non-small cell lung cancer cell lines A549 and H1650.Methods The effect of xanthohumol on the proliferation of A549 and H1650 cells was measured by CCK-8 method.The effect of xanthohumol on the apoptosis of A549 and H1650 cells was analyzed by flow cytometry.The expressions of apoptosis-related protein Bax,Bcl-xl and pro-caspase-3 in these two types of cells after 48 hof intervention by xanthohumol were tested by Western blot.Results xanthohumol could effectively inhibit the proliferation of A549 and H1650 cells in a dose-dependent manner.Its inhibitory effect on H1650 cells was stronger.Flow cytometry analysis showed that the apoptosis rate of these two types of cells increased with the concentration.The apoptosis rate induced by xanthohumol at the concentration of 5μmol·L^-1 was not different from that by the same concentration of cisplatin（P〉0.05）.The expressions of proteins related to apoptosis in the two cell lines varied with the dose of xanthohumol.Conclusion Xanthohumol may induce apoptosis of non-small cell lung cancer cells by regulating Bax,Bcl-xl,pro-caspase-3and the related genes.