频谱编码深度成像实验研究

Experimental Research on Depth-resolved Spectrally Encoded Imaging

频谱编码显微镜是用衍射光栅和光谱分析装置来获得显微图像.样品上不同的位置被不同的波长照明,通过对反射光光谱进行解码来得到空间信息.搭建了一个基于超连续光源和自制光谱仪的频谱编码显微成像系统,其横向分辨率为1.72±0.13μm(编码线方向)和1.26±0.08μm(垂直于编码线方向),测得不同横向位置处的轴向分辨率有差异.对离体猪肝组织不同部位进行了成像(可见血管、肝窦内皮细胞和肝细胞);对鸡心组织以10μm深度间隔进行成像,测得不同深度处结构信息不一样.结果表明,采用该频谱编码成像的方法能够进行高分辨的深度成像.

Spectrally encoded imaging uses a diffraction grating and a spectrum analyzer setup to obtain microscopic images. The different position on the sample is illuminated by different wavelength. Then the reflection light is decoded to obtain spatial information. In this letter, a spectrally encoded microscopy is described which is based on a super luminescent diode source and a home-built spectrometer. By imaging a 1951 USAF resolution target, the measured lateral resolutions were found to be 1.72±0.13 μm and 1.26±0.08 μm in the spectral and its vertical directions, respectively. The axial resolutions along the different lateral positions were measured to be unequal. The images of excised swine liver tissue were obtained at different locations. The veins, liver sinusoidal endothelial cells and hepatocytes can be visualized. The Chicken heart tissue was imaged at 10 μm per step along the depth direction beneath the surface, the images indicated the difference in the structure features at different depths. The results demonstrate high resolution, depth-resolved imaging capability by this method.