基于Th1细胞功能研究中药合地塞米松对哮喘大鼠pJAKs/pSTATs的影响

Effects of Traditional Chinese Medicine Combined with Dexamethasone on pJAKs/pSTATs in Asthmatic Rats Based on Th1 Cell Function

目的观察中药淫羊藿女贞子煎剂及提取物配伍地塞米松对哮喘大鼠肺组织中与Th1细胞信号通路相关的JAKs及STATs蛋白磷酸化表达的影响。方法 SPF级雄性大鼠,随机分为正常对照组(A组)、哮喘模型组(B组)、地塞米松组(C组)、淫羊藿女贞子煎剂组(D组)、地塞米松合煎剂组(E组)、淫羊藿女贞子提取物组(F组)、地塞米松合提取物组(G组)。卵蛋白致敏,激发建立大鼠哮喘模型,腹腔注射地塞米松和/或灌胃淫羊藿女贞子煎剂或提取物,免疫荧光法检测肺组织JAKs(JAK1、JAK2)及STATs(STAT1、STAT2、STAT4)蛋白的磷酸化表达。结果与A组比较,B组大鼠肺组织pJAK1、pSTAT1、pSTAT2蛋白表达均显著升高(均P<0.01),pJAK2、pSTAT4蛋白表达显著降低(均P<0.01);与B组比较,各给药组pJAK1、pSTAT1、pSTAT2蛋白表达显著降低(P<0.01或P<0.05),pJAK2、pSTAT4蛋白表达显著升高(均P<0.01);与C组比较,E组、G组能pJAK1、pSTAT1、pSTAT2的蛋白表达显著降低(P<0.01或P<0.05),pJAK2、pSTAT2的蛋白表达显著升高(P<0.01或P<0.05)。与单用地塞米松比较,地塞米松合煎剂或提取物组能显著性调整pJAK1、pJAK2、PSTAT1、pSTAT2、pSTAT4蛋白的表达。结论调节哮喘大鼠肺组织中与Th1细胞信号通路相关的JAKs/STATs可能是淫羊藿女贞子煎剂及提取物协同激素治疗哮喘的作用机制之一。

Objective： To study the effects of the decoction and extracts of Epimedium and Fructus Ligustri lucidi（EF） on JAKs/STATs protein phosphorylation which was connected with Thl cell signaling pathways in lung tissues of asthmatic rats atomized by dexamethasone. Methods： The SPF rats were randomly divided into seven groups,including normal control group （A group）,asthma model group （B group）,dexamethasone group （C group） ,EF decoction group （D group） ,EF decoction plus dexamethasone group （E group） ,EF extracts group（F group） ,and EF extracts plus dexamethasone group （G group）. Asthmatic rat model was dupheated by injection and atomization inhalation of OVA. And treatment was applied by intraperitoneal injection of dexamethasone and/ or gavage with EI7 decoction or EF extracts. The phosphorylation protein expressions of JAKs （including JAK1, JAK2） and STATs （including STAT1 ,STAT2,and STAT4） of lung tissues were detected in rats of all groups by the method of immunofluorescence. Results： Compared with A group,the protein expression of pJAK1 ,pSTAT1 and pSTAT2 in lung tissue were significantly increased （all P〈 0.01 ） in B group,and the protein expression of pJAK2 and pSTAT4 were also significantly decreased （all P〈 0.01 ）. Compared with B group,the protein expres- sion of pJAK1 ,pSTAT1 and pSTAT2 in five treatment groups were significantly decreased （P〈 0.05 or P〈 0.01 ）, and the protein expression of pJAK2 and pSTAT4 increased significantly （all P〈 0.01 ）. Compared with C group, E group,G group could significantly reduce the protein expression of pJAK1 ,pSTAT1 ,pSTAT2 （P 〈 0.05 or P 〈 0.01） ,and significantly increase pJAK2 and pSTAT2 protein expression （P〈 0.05 or P〈 0.01 ）. Compared with dexamethasone group, the EF decoction plus dexamethasone group or EF extracts plus dexamethasone group had a better effect on the protein expression of pJAK1, pJAK2, pSTAT1, pSTAT2, pSTAT4 protein. Conclusion： Combination of dexamethasone with EF may produce a synergistic interaction effect on asthma by adjusting JAKs/STATs protein phosphorylation expression which is connected with Thl cell signaling pathways.