淫羊藿苷对多发性硬化患者PBMC ER、GR mRNA表达的影响

Effects of icariin on expression of PBMC ER and GR mRNA in multiple sclerosis

目的探讨淫羊藿苷(ICA)对多发性硬化(MS)患者外周血单个核细胞(PBMC)中ERα、ERβ、GR mRNA表达水平的影响及其剂效关系。方法分离、提取MS患者及健康人PBMC并将其均分为空白对照组、雌激素组、高、中、低剂量ICA组以及对应浓度的空白血清对照组各8个亚组,培养后离心,以RQPCR法检测各组PBMC中ERα、ERβ、GR mRNA的表达量。结果 MS患者高、中剂量ICA及雌激素组ERα表达量较对照组明显升高,差异有统计学意义(P<0.05);MS患者高剂量ICA及雌激素组ERβmRNA表达量较对照组明显升高,差异有统计学意义(P<0.05)。MS患者和健康人中实验组与各对照组比较GR mRNA表达量无明显改变,差异无统计学意义(P>0.05)。MS患者与健康人高剂量ICA组比较,ERα、ERβmRNA表达量明显升高,差异有统计学意义(P<0.05)。结论 ICA可以提高MS患者PBMC中ERα、ERβmRNA的表达,具有拟雌激素样作用,但对MS患者PBMC中GR mRNA的表达无明显干预作用。

Objective To study the influence that icariin（ICA） exerts on the levels of ERα, ERβ and GR mRNA on PBMC in patients with multiple sclerosis （MS） and the relationship between dose and efficacy. Methods PBMC was isolated and extracted from MS patients and healthy volunteers, both of them were divided into 8 subgroups respectively including the blank control group, the estrogen group, three different doses of ICA groups and three correspondingly doses of blank serums control groups. The expression of ERα, ERβ and GR mRNA were detected by real-time quantitative PCR after all subgroups were cultured and centrifuged. Results Compared with the control group respectively, ERa mRNA expressions in ICA groups of high-, middle-doses and estrogen group of MS patients were significantly increased, and ER- mRNA expressions were significantly increased in ICA groups of high-dose and estrogn groups of MS patients. There was no significant difference between MS patients and healthy people on levels of GR mRNA expression compared with the control groups. Compared with the healthy people group respectively, there were significant differences on ERα and ERβ mRNA expressions in MS patients groups with high-doses of ICA. Conclusion ICA has estrogenic effect and increases expressions of ERα and ERβ mRNA on PBMC in MS patients, but it has no effects on expressions of GR mRNA on PBMC in patients with MS.