春秋两季不同地区猪源耐药大肠杆菌β-内酰胺酶及16SrRNA甲基化酶基因检测及分析

Detection and Analysis on β-lactam Enzyme and 16S rRNA Methylase Genes in Swine Resistance E. coli Isolated from Different Areas of Xinjiang in Spring and Autumn

为了解新疆春秋两季不同地区耐药猪源大肠杆菌携带β-内酰胺酶和16S rRNA甲基化酶基因及其共存情况。通过PCR方法对克拉玛依地区142株春季猪源耐药菌、9株克拉玛依秋季猪源耐药菌、78株昌吉地区春季猪源耐药菌和52株昌吉地区秋季猪源耐药菌进行β-内酰胺酶(blaTEM、blaCMY-2、blaLAP-1、blaKPC、blaSHV和blaOXA)和16S rRNA甲基化酶(arm A和rmt B)基因检测,并对检出耐药基因的PCR产物进行测序。结果显示:克拉玛依春季猪源耐药菌携带的β-内酰胺酶基因为blaTEM(142/142,100%)、blaOXA(16/142,11.3%)、blaCMY-2(7/142,4.9%)和blaLAP-1(1/142,0.7%);克拉玛依秋季猪源耐药菌携带的β-内酰胺酶为blaTEM(9/9,100%)和blaOXA(1/9,11.1%),克拉玛依春秋两季猪源耐药菌均未检测出16S rRNA甲基化酶基因;昌吉地区春季猪源耐药菌携带的β-内酰胺酶基因为blaTEM(78/78,100%)、blaOXA(9/78,11.5%)、blaCMY-2(3/78,3.8%)和16S rRNA甲基化酶基因rmt B(1/78,1.3%);昌吉地区秋季猪源耐药菌携带的β-内酰胺酶基因为blaTEM(52/52,100%)、blaOXA(4/52,7.7%)、blaCMY-2(3/52,5.8%)和blaSHV(1/52,1.9%),未检测出16S rRNA甲基化酶基因。上述结果表明春秋两季不同地区猪源大肠杆菌blaTEM检出率均为100%;其次不同地区春季猪源大肠杆菌对blaOXA检出率均高于秋季猪源大肠杆菌。产生β-内酰胺酶及16S rRNA甲基化酶基因是猪源菌对β-内酰胺类及氨基糖苷类抗菌药物耐药的主要原因之一,养殖场应合理使用抗菌药物,加强对产生β-内酰胺酶及16S rRNA甲基化酶基因的监控。

In order to investigate 13-1actam enzyme and 16S rRNA methylase genes in resistant swine E. coli isolated from different areas of Xinjiang in spring and autumn and their coexistence. 13-1actamase （ baTEM , blacM,l_2, blaLAp_1 , blaKPC, blassvand blaoA ） and 16S rRNA methylase genes （arraA and rmtB） were detected in 142 （spring） and 9 （autumn） swine resistant E. coli isolated from Karamay area, 78 （spring） and 52 （autumn） swine resistant E. coli isolated from Changji area by PCR, respectively. And PCR products of the resistant genes were sequenced. The results showed that blaTEM （ 142/142, 100%）, blaoxA （ 16/142, 11.3%）, blacMY_2 （7/142, 4.9%） and blaLAp_l （1/142, 0.7%） genes were mainly detected in swine resistant E. coli isolated from spring in Karamay area; blaTEM（9/9, 100%） and blaoxA（ 1/9, 11.1%） genes were mainly detected in swine resistant E. coli isolated from Karamay area in autumn; 16S rRNA methylase genes were not detected in swine resistant E. coli isolated from Karamay area in spring and autumn. β-lactamase genes as blaTM （ 78/78, 100% ）, blaoxA （ 9/78, 11.5% ）, blacy_2 （ 3/78, 3.8% ） and 16S rRNA methylase gene as rmtB （1/78, 1.3%） were mainly detected in swine resistant E. coli isolated from Changji area in spring ; blawEM（52/52, 100%） , blaoxA（4/52, 7.7%） , blacMy_2（3/52, 5.8%） and blasHv（ 1/52, 1.9%） genes were mainly detected in swine resistant E. coli isolated from Changji area in autumn, and 16S rRNA methylase genes were not detected. These results indicated that detection rate of blaTEM was 100% in swine resistant E. coli isolated from different areas in spring and autumn. That detection rate of blaoxA in swine resistant E. coli isolated from different areas in spring was higher than that in autumn. Bacteria carry β-1actamase and 16S rRNA methylase genes were one of the main resistant reasons to antimicrobinl drugs. So we should regulate the use of antibiotics, strengthen and monitor E. coli carrying the β-lactamase and 16S rRNA methvlase genes.