摘要
To achieve a stable, sensitive, and high-efficiency biological probe, a novel NaYF4：Yb,Er nanocrystals/TiO2 inverse opal composite film was designed by self-assembly and solvent evaporation methods. 32-fold enhanced upconversion（UC） emission was investigated under 980 nm excitation. According to size-dependency, excitation power density-dependency as well as photonic stop band（PSB）-dependency upconversion spectra, the enhancement mechanism of the composite film was put down to the stochastical diffraction of IOPCs multi-layered structure to the excitation laser. On the basis of the enhancement effect of the composite film, energy transfer between upconversion nanoparticles（UCNPs） and quantum dots（QDs）, and the sensitive sensing of CdTe QDs on mercury, the UC composite film was used for sensing of Hg^2＋ in serum. The solid sensor as a mercury detector owns lots of superiorities such as feasible operation, good linear relationship（R=0.997）, low limit of detection（70.5 nmol/L） and thus may have broad prospects in the biosensing field.
To achieve a stable, sensitive, and high-efficiency biological probe, a novel NaYF4：Yb,Er nanocrystals/TiO2 inverse opal composite film was designed by self-assembly and solvent evaporation methods. 32-fold enhanced upconversion（UC） emission was investigated under 980 nm excitation. According to size-dependency, excitation power density-dependency as well as photonic stop band（PSB）-dependency upconversion spectra, the enhancement mechanism of the composite film was put down to the stochastical diffraction of IOPCs multi-layered structure to the excitation laser. On the basis of the enhancement effect of the composite film, energy transfer between upconversion nanoparticles（UCNPs） and quantum dots（QDs）, and the sensitive sensing of CdTe QDs on mercury, the UC composite film was used for sensing of Hg^2＋ in serum. The solid sensor as a mercury detector owns lots of superiorities such as feasible operation, good linear relationship（R=0.997）, low limit of detection（70.5 nmol/L） and thus may have broad prospects in the biosensing field.
基金
Project supported by National Natural Science Foundation of China(11374044)
Fundamental Research Funds for the Central Universities(3132016333,3132017058)
Doctor Start-up Funds of Liaoning Province(201601067)
China Postdoctoral Science Foundation(2016M591420)
