摘要
目的探讨不同浓度丁酸钠(NaB)对干扰素调节因子5(IRF5)基因表达的影响。方法体外培养人肺腺癌细胞A549和人胚肾细胞HEK293,分别用去离子水(对照组)和浓度为1.0、2.5和5.0mmol/L的NaB处理细胞(实验组),采用双荧光素酶报告基因技术检测IRF5基因启动子活性,采用RT-qPCR及Western blot法检测IRF5基因mRNA及蛋白表达水平。结果与对照组相比,实验组NaB可降低A549细胞及HEK293细胞中IRF5基因启动子活性、抑制A549细胞中IRF5基因mRNA及蛋白的表达(P<0.05或P<0.01)。随着NaB处理浓度的增加,IRF5基因启动子活性、mRNA及蛋白表达均呈下降趋势。结论 NaB对体外IRF5基因表达具有抑制作用。
Objective To investigate the effect of different concentrations of sodium butyrate(NaB)on the expression of interferon regulatory factor 5(IRF5)gene.Methods Human lung adenocarcinoma cell line A549 and human embryonic kidney cell HEK293 were cultured in vitro.The cells were treated with deionized water(group C)and NaB(group A)in the concentrations of 1.0,2.5and 5.0 mmol/L,respectively.The promoter activity of IRF5 was detected by double luciferase reporter gene technique.The mRNA and protein expressions of IRF5 gene were detected by RT-qPCR and Western blot.Results Compared with group C,NaB in group A decreased the promoter activity of IRF5 gene in A549 cells and HEK293 cells,and inhibited the mRNA and protein expressions of IRF5 in A549 cells(P〈0.05 or P〈0.01).With the increase of NaB concentration,IRF5 gene promoter activity,mRNA and protein expression were decreased.Conclusion NaB in vitro inhibits the expression of IRF5 gene.
出处
《江苏医药》
CAS
2017年第8期533-536,共4页
Jiangsu Medical Journal
基金
国家自然科学基金(30570863
30872804
81170661)
教育部博士点基金(20113234110010)
关键词
丁酸钠
干扰素调节因子5
Sodium butyrate
Interferon regulatory factor 5