浓香型白酒糟醅中农药降解菌的筛选及多样性分析

Selection and diversity of pesticide-degrading bacteria isolated from Zaopei of Luzhou-flavor Liquor

通过富集培养和平板初筛,从浓香型白酒糟醅中分离筛选可降解农药的微生物菌株,并进行16SrRNA基因序列系统发育分析。结果显示:52株分离菌株中,可降解丙环唑、莠去津和2,4D丁酯的菌株分别为25,10,17株,其中51株菌分属于Bacillus、Lysinibacillus、Pseudomonas、Acinetobacter、Brevibacillus和Stenotrophomonas 6个属的22个模式菌株,1株菌与B.thuringiensis的序列相似度为97.3%,可能是该菌株的变种;Bacillus是可以降解3种农药的共有属和优势属(37株,占总菌数的71.2%),Stenotrophomonas和Brevibacillus分别为降解莠去津和2,4D丁酯的特有属。

The pesticide-degrading bacteria was isolated and selected fromZaopei of Luzhou-flavor Liquor by the enrichment culture and plate separation technology,then the phylogenetic analysis of these strains were carried out by 16 SrRNA gene sequence.The results showed that 52 strains were isolated from Zaopei of Luzhou-flavor Liquor,and 25 strains had degradative capability for Propiconazol,and 10,17 had degradative capability for,Atrazine,2,4-D butyl ester respectively.51 strains belonged to 22 species of 6genera（Bacillus,Lysinibacillus,Pseudomonas,Acinetobacter,Brevibacillus,Stenotrophomonas）,and 1isolate was presumed to be variety of Bacillus thuringiensis（with 97.3% sequence similarity）.Bacillus was the shared and dominant genus for,degradation three kinds of pesticides（Propiconazol,Atrazine,2,4-D butyl ester）with 37strains（accounting for 71.2% of 52strains）.Stenotrophomonas and Brevibacillus was the specific genera for Atrazine and 2,4-D butyl ester degradation respectively.