冷藏期间草鱼和鲢鱼鱼片特征生物胺变化差异

Study on the difference of Characteristic Biogenic Amines in Grass and Silver Carp Fillets during cold Storage

旨在明确草鱼和鲢鱼鱼片冷藏过程中生物胺的动态变化规律以及其特征生物胺变化差异,测定分析了4℃贮藏条件下草鱼和鲢鱼片pH、挥发性盐基氮(TVB-N)、菌落总数(TVC)、感官评价以及生物胺含量的动态变化。结果表明,随着贮藏时间的延长,TVB-N、TVC呈明显上升趋势;草鱼和鲢鱼鱼片冷藏过程中苯乙胺、腐胺、酪胺及8种生物胺总量变化明显,且草鱼和鲢鱼鱼片中各生物胺显著变化的时间点分别为第10天和第8天。草鱼和鲢鱼片生物胺含量的差异变化主要发生在腐败后期,在贮藏第12天,草鱼、鲢鱼片间腐胺和酪胺含量差异显著(P<0.05),其含量分别为(23.39±0.40),(44.46±1.88)mg/kg和(25.01±1.85),(50.84±1.50)mg/kg。经相关性分析显示,苯乙胺、腐胺、酪胺与草鱼片品质指标间显著相关(P<0.05),可作为草鱼品质的监测指标;苯乙胺、腐胺、酪胺、尸胺与鲢鱼片品质指标间显著相关(P<0.05),可作为鲢鱼片品质的评价指标。且冷藏期间草鱼和鲢鱼鱼片关键腐败时间点的酪胺和8种总生物胺的含量分别相近,可作为草鱼和鲢鱼片品质评价的共同指标。

In order to investigate the change of biogenic amines, and the correlation between quality indices and characteristics of biogenic amines, the change of pH, TVB-N, TVC, sensory evaluation and biogenic amines of grass carp and silver carp fillets during cold storage at 4℃ were detected. The results showed that TVBN and TVC increased with the cold storage time; phenethylamine, putrescine, tyramine and the total biogenic amines of grass carp and silver carp fillets changed obviously. All kinds of biogenic amines of grass carp and silver carp fillets significantly changed at the 10^th and 8^th day. Significant differences （P〈0.05） existed in putrescine and tyra-mine of grass carp and silver carp fillets after corruption. The contents of putrescine and tyramine in grass carp fillets and silver carp fillets respectively were （ 23.39±0.40） mg/kg and （ 44.46±1.88） mg/kg, （25.01±1.85） mg/kg and （50.84±1.50） mg/kg at 12^th day. Correlation analysis showed that there was significant correlation（P 〈 0.05 ） between phenylethylamine, putrescine, tyramine and quality indices in grass crap fillets. Quality indices were significantly correlated （ P〈 0.05） with phenylethylamine, putrescine, eadaverine, tyramine in silver crap fillets. Therefore, phenylethylamine, putrescine, and tyramine could be used as the quality evalua- tion indices of grass carp fillets. Moreover, the quality of sliver carp fillets could be evaluated by phenylethylamine, putrescine, cadaverine, tyramine. In addition, the critical value of tyramine and total biogenic amines in grass carp fillets and silver carp fillets during the chilled storage respectively were similar, which could also be used as the quality evaluation indicators of grass carp and silver carp fillets.