香烟烟雾染毒对雄性大鼠睾丸组织一氧化氮水平的影响

The effect of cigarette smoke exposure on nitric oxide level in testis of male rats

目的探讨香烟烟雾染毒对大鼠睾丸组织结构及睾丸组织一氧化氮(NO)水平的影响。方法清洁级成年雄性SD大鼠160只,分为对照组和低、中、高剂量染毒组,各组大鼠分别染毒2、4、6、8、12周,每组10只。染毒组采用呼吸道静式染毒,每天1次,每次0.5h。观察大鼠睾丸组织结构,检测各组大鼠体质量及睾丸组织NO水平。结果染毒4、6、8、12周时,中、高剂量染毒组大鼠体质量与对照组和低剂量组相比均明显降低(P<0.05),染毒8、12周时,高剂量染毒组大鼠体质量与中剂量组相比明显降低(P<0.05),并且随着染毒剂量的增加,降低更加明显。染毒2、4、6、8周时,高剂量组大鼠睾丸组织NO水平与对照组和低、中剂量组相比均明显升高(P<0.05);染毒8周时,中剂量组大鼠睾丸组织NO水平与对照组相比明显升高(P<0.05);染毒12周时,低、中、高剂量组大鼠睾丸组织NO水平与对照组相比均明显升高(P<0.05),中、高剂量组大鼠睾丸组织NO水平与低剂量组相比明显升高(P<0.05)。结论香烟烟雾染毒可致雄性大鼠睾丸组织NO水平升高,导致雄性大鼠睾丸组织损伤。

Objective To explore the effect of cigarette smoke exposure on testis structure and nitric oxide （NO） level in testis of rats. Methods A total of 160 adult male SD rats were divided into control group and low,medium, high dose groups. The smoke-exposed rats were respectively exposed to the smog for the periods of 2,4,6,8 and 12 weeks, 10 rats in each group. The smoke-exposed rats were exposed to cigarette smoke for 0.5 h per day. The testicular tissue structure was observed and the body mass and the NO level of testis were measured. Results Compared with the control group and low dose group,the body mass of the rats was significantly lower in the medium and high dose groups exposed for 4,6,8,12 weeks （P〈0.05）. Compared with the medium dose groups,the body mass of the rats was significantly lower in the high dose group exposed for 8 weeks and 12 weeks （P〈 0.05）. And with the increase of exposure dose,the reducing was even more obvious. Compared with the control group and low and medium dose groups,NO level in testis of rats was significantly increased in the high dose groups exposed for 2,4,6,8 weeks （P〈 0.05）. Compared with the control group,NO level in testis of rats was significantly increased in the medium dose groups exposed for 8 weeks （P〈0.05）. Compared with the control group,NO level in testis of rats was significantly increased in the low,medium and high dose groups exposed for 12 weeks （P〈0.05） ;and compared with the low dose group,NO level in testis of rats was significantly increased in the medium and high dose groups（P〈0. 05）. Conclusion Cigarette smoke exposure may have impacts on testis tissue and lead to the increase of NO level in testis of male rats.