摘要
目的探索改良的人心房肌细胞分离方法。方法采用两步酶消化法急性分离人心房肌细胞,采用膜片钳全细胞记录技术记录小电导钙激活钾通道。结果该分离方法可获得数量较多的心房肌细胞,窦性心律患者可获得完整有横纹细胞320±30,慢性房颤患者可获得完整有横纹细胞230±20,比较差异有统计学意义(P<0.01)。该方法可获得大量形态完整,横纹清晰的单个人心房肌细胞,且能在所分离的心房肌细胞上记录到典型的小电导钙激活钾通道电流。结论该试验所采用的分离方法简便,稳定有效,可获得细胞数量多和细胞质量好的单个人心房肌细胞。
Objective To study an improved isolated method of single human atrial myocytes. Methods Enzyme digestion method was used to isolate single myocytes from human atrial and whole-cell patch clamp technique was used to record small conductance calcium activated potassium current. Results This method obtained a large number of atrial myocytes. The total amount of atrial myocytes in SR group was 320±30 while AF group was 230±20 and the difference was statistically signifieant（P〈0.01）. In this study,a large number of simple and striated single atrial myocytes were obtained,and a typical small-conductance calcium-activated potassium channel current was recorded on the isolated atrial myocytes. Conclusion The established isolated method is sim- ple,stable and effective. We can acquire a large amount of single atrial myoeytes with good quality.
作者
王笑宇
范忠才
周伟
余奕言
周涛
李妙龄
Wang Xiaoyu Fan Zhongcai Zhou Wei Yu Yiyan Zhou Tao Li Miaoling(Department of Cardiology, the Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China Key Laboratory of Medical Electrophysiology ,Ministry of Education of China, Institute of Cardiovascular Research, Southwest Medical University, Luzhou , Sichuan 646000, China)
出处
《重庆医学》
CAS
北大核心
2017年第14期1941-1943,1946,共4页
Chongqing medicine
基金
国家自然科学基金资助项目(81470022)
四川省教育厅重点项目(16ZA0192)
泸州市-泸州医学院联合项目(20140975)
关键词
电学
肌细胞
心脏
人心房肌
分离方法
膜片钳
离子通道
eleetricity
myocytes,cardiac
human atria myocytes
isolated method
patch-clamp technique
ionic channel
