结核分枝杆菌higA基因的扩增及生物信息学分析

Analysis of amplification and bioinformatics on mycobacterium tuberculosis protein higA

目的对结核分枝杆菌的higA基因进行扩增,同时利用生物信息学方法分析其编码蛋白的结构和功能。方法从结核分枝杆菌标准株H37Rv基因组DNA中扩增higA基因,对扩增产物进行测序。利用生物信息学网站和软件分析higA蛋白的理化性质、信号肽、空间结构、抗原表位等。结果 higA基因扩增产物与预期一致,大小为450bp。higA蛋白共149个氨基酸,理论等电点7.93,脂溶性系数94.30,不稳定系数36.57,预测该蛋白为稳定蛋白。higA蛋白无信号肽,含10个磷酸化位点和多个潜在的抗原表位。结论成功扩增出结核分枝杆菌higA基因。

Objective To amplify the higA gene from the Mycobacterium tuberculosis,and to analyze the structure and function of their encoded proteins by using bioinformatics. Methods Total DNA was extracted from Mycobacterium tuberculosis. PCR of higA was performed and the products were sequenced. The biological features of the higA protein including, its physical and chemical properties,signal peptide,spatial structure and epitopes were analyzed by using software online. Results The PCR prod- ucts of higA were 450 bp in length,which were consistent with the expected size. The higA protein consisted of 149 amino acids and had the following characteristics：a theoretical isoelectric point of 7.93, a fat-soluble factor of 94.30,and instability coefficient of 36.57. The higA protein had no signal peptide,eontaining 10 phosphorylation sites and multiple potential epitopes. Conclusion Mycobaeterium tuberculosis higA gone can be amplified by PCR and the characteristics of higA protein is identified.