摘要
[目的]通过测定岭南牛线粒体D-loop全序列来了解岭南牛的母系起源及遗传多样性。[方法]采用PCR扩增、测序及生物信息学方法。[结果]通过对30头岭南牛mtDNA D-loop序列分析,共发现62个变异位点和19种单倍型,核苷酸多样度(Pi)为0.0260,单倍型多样度(Hd)为0.8920,表明岭南牛遗传多样性丰富。构建的NJ系统进化树表明岭南牛有普通牛和瘤牛2种母系起源。[结论]岭南牛属于南方牛类型,受瘤牛的影响较大,具有瘤牛和普通牛的种质特征。
[Objective] The aim of the research was to study the maternal origin and genetic diversity of Lingnan cattle. [Method] The complete mtDNA D-loop regions of 30 Lingnan cattle were analyzed using PCR, sequencing and bioinformatics methods. [Result] The results showed that 62 variations were detec- ted in the complete mtDNA D-loop regions of 30 Lingnan cattle, which defined 17 haplotypes. The nucleo- tide diversity （Pi） was 0. 0260, and the haplotype diversity （Hd） was 0. 8920, indicating an abundant ge- netic diversity in Lingnan cattle. The neighbor-joining tree showed that Lingnan cattle had two types of maternal origins from Bos taurus and Bos indicus. [Conclusion] Lingnan cattle, belonging to the southern cattle type in China, which was mainly affected by Bos indicus, had two types of maternal origins from Bos taurus and Bos indicus.
作者
赵晓诚
党瑞华
黄永震
蓝贤勇
宋恩亮
陈宏
左自意
雷初朝
ZHAO Xiao-cheng DANG Rui-hua HUANG Yong-zhen LAN Xian-yong SONG En-liang CHEN Hong ZUO Zhi-yi LEI Chu-zhao(College of Animal Science and Technology, Northwest A＆F University,gangling, Shaanxi 712100, China Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, J inan , Shandong , 250100, China Shannxi Yi fa Ecological Animal Husbandry Developing co. ltd, Shannxi shah yang 726499)
出处
《中国牛业科学》
2017年第2期1-3,共3页
China Cattle Science
基金
国家肉牛牦牛产业技术体系资助(CARS-38)
