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实时荧光PCR检测非洲猪瘟病毒 被引量:3

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摘要 非洲猪瘟(ASF)目前已对全球多个发病国家的养猪业造成了巨大的经济损失,是我国明确规定须重点防范的外来动物疫病之一,其对我国的威胁也与日俱增。本实验采用北京世纪元亨非洲猪瘟实时荧光PCR试剂盒,重点对来源于四川省阿坝、广元、巴中、达州、广安等边境高危地区的规模场及散养户的猪组织病料进行了检测,结果表明97份组织样本均为ASFV抗原阴性,四川境内尚未发现ASFV感染。
出处 《猪业科学》 2017年第5期48-49,共2页 Swine Industry Science
基金 定点监测与流调子项目 国家动物疫病防治与监测项目
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  • 1Dixon L K, Escribano J M, Martins C, et al. Asfarviridae. In : virus taxonomy, eighth report of the ICTV[ M]. London : Elsevier/Aca demic Press, 2005,135-143.
  • 2German A, All A,Jose S, et al. African swine fever virus polypro- teins pp220 and pp62 assemble into the core shell[J]. Virology, 2002,76 (24) : 12473- 12482.
  • 3Lopez-Otin C, Freije J M P,Parra F, et al. Mapping and sequence of the gene coding for protein p72, the major capsid protein of Af- rican swine fever virus[J]. Virology, 1990,175(2) :477-484.
  • 4Office International des Epizooties. African swine fever,in manu- al of diagnostic tests and vaccines for terrestrial animals[M]. 5th ed. Paris : Office International des Epizooties, 2004,1178.
  • 5PENRITH M L, THOMSON G R, BASTOS A S S. African swine fever[M]//COETZER J A W,TUSTIN R C. Infectious Diseases of Livestock. Southern Africa: Oxford University Press,2005: 1087-1119.
  • 6LUBISI B A,BASTOS A D, DWARKA R M,et al. Molecular epidemiology of African swine fever in East Africa[J]. Arch Virol, 2005,150(12): 2439-2452.
  • 7World Organization for Animal Health. Manual of diagnostic tests and vaccines [online]. Paris: OIE, 2004. African swine fever. Available at: http://www. oie. int/eng/normes/mmanual/A_00035, htm. Accessed 4 Dec. 2006.
  • 8FERNANDEZ DE MARCO M, SALGUERO F J, BAUTISTA M J ,et al. An immunohistochemical study of the tonsils in pigs with acute African swine fever virus infection[J]. Res Vet Sci, 2007,83(2) : 198-203.
  • 9PAN I C,SHIMIZU M, HESS W R. African swine fever: microplaque assay by an immunoperoxidase method[J]. Am J Vet Res,1978,39(3) :491-497.
  • 10MALMQUIST W A, HAY D. Haemadsorption and cytopathic effect produced by African swine fever virus in swine bone marrow and huffy coat cultures[J]. Am J Vet Res, 1960,21: 104-108.

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