巨细胞病毒感染对妊娠妇女调节性免疫细胞的作用研究

Effect of cytomegalovirus infection on regulatory immune cells in pregnant patients

目的研究巨细胞病毒(CMV)感染对妊娠患者调节性免疫细胞的作用。方法收集2015年5月-2016年9月来医院就诊妊娠患者的临床资料,抗原pp65检测阴性的30例患者为对照组;抗原pp65检测阳性的30例患者为CMV感染组,采用梯度离心方法分离患者外周血淋巴细胞,CD_4、CD_(25)和Foxp_3流式抗体标记调节性T细胞(Treg细胞);CD_(19)和TGF-β流式抗体标记调节性B细胞(Breg细胞);CD_4、CXCR_5和Foxp_3流式抗体标记滤泡调节性T细胞(Tfr细胞)。结果 CMV感染组CD_4^+CD_(25)^+Foxp_3^+的Treg细胞比例显著低于对照组,下降了31.9%,差异有统计学意义(P<0.05);CMV感染组CD_(19)^+TGF-β^+的Breg细胞比例显著低于对照组,下降了78.5%,差异有统计学意义(P<0.05);CMV感染组中CD_4^+CXCR_5^+Foxp_3^+的Tfr细胞比例显著低于对照组,下降了65.1%,差异有统计学意义(P<0.05)。结论 CMV感染后,妊娠患者体内Treg细胞、Breg细胞和Tfr细胞等调节性免疫细胞的比例均显著下降,可能对胎儿的生长发育造成影响。

OBJECTIVE To observe the effect of cytomegalovirus（CMV）infection on regulatory immune cells in pregnant patients.METHODS The clinical data were collected from the pregnant patients who were treated in the hospital from May 2015 to Sep 2016;30patients who were detected negative for antigen pp65 were assigned as the control group,and 30 patients who were detected positive for antigen pp65 were set as the CMV infection group.The peripheral blood lymphocytes were isolated by using gradient centrifugation method,CD4,CD25,and Foxp3 streaming antibodies were used to label regulatory T cells（Treg cells）;CD19and TGF-βstreaming antibodies were used to label regulatory B cells（Breg cells）;CD4,CXCR5 and Foxp3 streaming antibodies were used to label follicular regulatory T cells（Tfr cells）.RESULTS The proportion of the patients with CD4＋CD25＋Foxp3＋Treg cells of the CMV infection group was significantly lower than that of the control group,with a decrease of 31.9%（P〈0.05）.The proportion of the patients with CD19＋TGF-β＋Breg cells of the CMV infection group was significantly lower than that of the control group,with a decrease of 78.5%（P〈0.05）.The proportion of the patients with CD4＋CXCR5＋Foxp3＋Tfr cells of the CMV infection group was significantly lower than that of the control group,with a decrease of 65.1%（P〈0.05）.CONCLUSIONThe proportion of the pregnant patients with in vivo regulatory immune cells such as Treg cells,Breg cells,and Tfr cells are significantly reduced after they had the CMV infection,which may have adverse effect on fetal growth.