纳米碳对离体培养条件下几种植物生长及分化的影响

Effects of Nano Carbon on the Growth and Differentiation of Several Plants in Vitro Culture

以食用百合、红掌、大花蕙兰及金昌枣的组培苗为材料,研究不同浓度的纳米碳对几种植物外植体生长及分化的影响。结果表明,MS+6-BA 0.8 mg/L+NAA 0.2 mg/L+纳米碳0.10 g/L,促进百合鳞茎增殖,当纳米碳为0.50 g/L时有利于百合苗的生长及生根;MS+6-BA 1.5 mg/L+IBA 0.2 mg/L+纳米碳0.04-0.06 g/L适用于红掌腋芽外植体愈伤组织诱导和分化,诱导率达90%以上,分化率达97%;加入纳米碳1.0-2.0 g/L时能够明显抑制大花蕙兰原球茎继代培养中的褐化现象;MS+6-BA 1.5 mg/L+IBA 0.5 mg/L+纳米碳0.05 g/L,利于金昌枣芽增殖,当纳米碳为0.15 g/L时,芽长势健壮。

This work is to study the effects of different concentrations of nano carbon on growth and differentiation of several plants＇ explants. The test tube seedling of edible lily,Anthurium andraeanum,Cymbidium hybridum,and Jinchang jujube as experimental materialwas cultured on MS medium with different concentrations of nano carbon. Results showed that,MS ＋ 6-BA 0.8 mg/L ＋ NAA 0.2 mg/L ＋nano carbon 0.10 g/L was conducive to the multiplication of lily bulb with the multiplication coefficient 2.11;when the concentration of nanocarbon was 0.50 g/L,the growth and rooting of lily in vitro was significantly better. The optimal medium for induction and differentiation ofAnthurium ‘s axillary buds was MS＋ 6-BA 1.5 mg/L ＋ IBA 0.2 mg/L ＋ nano carbon 0.04 to 0.06 g/L,the induction rate was over 90% and thedifferentiation rate was 97%. The addition of nano carbon 1.0 to 2.0 g/L obviously inhibited the browning of C. hybridum protocorm subculture.The optimal medium for the multiplication cultivation of Jinchang jujube was MS ＋ 6-BA 1.5 mg/L ＋ IBA 0.5 mg/L ＋ nano carbon 0.05 g/L,themultiplication coefficient reached 9.76;bud growth was robust when the concentration of nano carbon was 0.15 g/L.