食品中副溶血性弧菌检测能力验证分析

Analysis of Proficiency Testing for Food-borne Vibrio Parahemolyticus

目的通过参加CNAS(中国合格评定国家认可委员会)食品中副溶血性弧菌检测能力验证计划,对实验室检测检测能力及质量进行确认。方法采用国标法及实时荧光PCR法对标识为VP-162考核样品及超市随机抽取的50份动物性海水产品进行副溶血性弧菌定性检测,通过检测结果比较进行方法评价。结果 VP-162考核样品中检出副溶血性弧菌,考核结果为满意。随机抽取的50份动物性海水产品,干制生化试剂及API20E均检出6份(检出率12.0%),全自动微生物生化鉴定系统检出5份(检出率10.0%),实时荧光PCR法检出8份(检出率16.0%)。结论实时荧光PCR检测结果准确、灵敏、快速,在运用国标法进行检测过程中,采用生化鉴定试剂与全自动微生物生化鉴定系统分别进行鉴定时,其鉴定结果存在一定差异。

Objective To verify the laboratory test capacity and quality by participating in the CNAS （China Na- tional Accreditation Service for Conformity Assessment） proficiency testing program for food - borne Vibrio para- hemolyticus. Methods A blind sample labelled as VP - 162 and 50 marine animal food samples were collected randomly from supermarkets and were tested for Vibrio parahemolyticus by the national standard methods and real -time PCR separately; the test results were compared and evaluated. Results Vibrio parahemolyticus was detected from the VP- 162 sample, complying with the conformity requirement. Among the 50 marine animal food samples, Vibrio parahemolyticus was detected from six （ 12. 0% ） by using dry bio - reagents and API20E each, Vibrio parahemolyticus was also detec ted from five （ 10. 0% ） by using an automatic biochemical identifi- cation system and from eight （ 16. 0% ） by real - time PCR. Conclusion The real - time PCR method is accu- rate, sensitive and rapid. When the national standard method was used, test results obtained with bio - reagents and the automatic biochemical identification system were not completely consistent.