前列腺癌组织紧密连接蛋白-4表达及与生化复发的关系

Relationships of Expressionof Claudin-4 and Biochemical Recurrence in Prostate Cancer

目的探讨紧密连接蛋白-4(Claudin-4)在前列腺癌中的表达及与前列腺癌生化复发之间的关系。方法应用S-P法检测79例前列腺癌和45例前列腺增生组织Claudin-4表达状况,分析其与前列腺癌临床病理特点之间的关系,对前列腺癌随诊结果采用Kaplan-Meier生存分析5年前列腺癌患者生化复发与Claudin-4表达之间的关系;结果前列腺癌组织和前列腺增生组织Claudin-4表达阳性率分别为64.56%(51/79)和33.33%(15/45),差异有统计学意义(P均<0.01)。Claudin-4阳性表达在前列腺癌TNM分期Ⅰ~Ⅱ期和Ⅲ~Ⅳ期分别为46.34%和84.21%,在Gleason评分<7分≥7分患者分别为57.14%和72.97%,两者比较,差异有统计学意义(P<0.05)。随访60个月内生化复发45例,复发率为56.96%,生化复发的患者中Claudin-4表达阳性39例,Claudin-4表达阴性6例,两组间生化复发率比较差异有统计意义(P<0.01)。前列腺癌患者PSA结果进行Kaplan-Meier生存分析显示Claudin-4阳性患者5年生化复发率高于阴性表达患者。结论前列腺癌组织Claudin-4表达增高,其异常表达与前列腺癌临床分期和Gleason评分及5年生化复发相关,联合检测Claudin-4可能有助于前列腺癌预后评估。

Objective To investigate the expression of Claudin-4 in prostate cancer tissue and its relationship with biochemical recurrence of prostate cancer. Methods S-P method was used to detect the expression of Claudin- 4 in 79 cases with prostate cancer and 45 cases with benign prostatic hyperplasia, then the relationship between its expression withthe clinical and pathological features was analyzed. Kaplan-Meier survival analysis was used to analyze 5-year biochemical recurrence in patients and the follow-up results was assessed with the expression of Claudin-4. Results The positive rate of Claudin-4 expression in prostate cancer and benign prostatic hyperplasia was64.56% （51/79） and 33.33%（15/45）, respectively, with a significant different between them （P〈0.01）. The positive expres- sion rates of Claudin-4 in tissues at TNM stage Ⅰ-Ⅱ and Ⅲ-Ⅳ were 46.34% and 84.21% and intissues from the Gleason score 〈7 and ≥7 were 57.14% and 72.97%,both with a significant difference （P〈0.05）. In a follow-up of 60 months, 45 cases hadbiochemical recurrence, with a recurrence rate of 56.96%;among them, 39 cases had posi- tive expression of Claudin-4, 4 cases with negative Claudin-4 expression; the difference in recurrence rate was sta- tistically significant（P〈0.01）. Kaplan-Meier survival analysis showed that Claudin-4-positive expression patients had a higher 5-year biochemical recurrence rate than patients with Claudin-4 negative expression. Conclusion Claudin-4 expression was increased in prostate cancer, the increase was related to the clinical stage and Gleason score and biochemical recurrence of prostate cancer. Detection of Claudin-4 may contribute to prognosis of prostate cancer.